Measurement of gap junctional communication by fluorescence activated cell sorting.
Measurement of gap junctional communication by fluorescence activated cell sorting.
复制标题
通过荧光激活细胞分选测量间隙连接通讯。
DOI：
10.1007/bf02631304
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Atkinson,MM
中科院分区：
文献类型：
--
作者：
Kiang,DT;Kollander,R;Lin,HH;LaVilla,S;Atkinson,MM
Cell-to-cell communication via gap junctions has played a fundamental role in the orderly development of multicellular organisms. Current methods for measuring this function apply mostly to homotypic cell populations. The newly introduced Fluorescence Activated Cell Sorting (FACS) method, albeit with some limitations, is simple, reliable, and quantitative in measuring the dye transfer via gap junctions in both homotypic and heterotypic cell populations. In the homotypic setting, the result in dye transfer from the FACS method is comparable to the scrape-loading and microinjection methods. Using this FACS method, we observed a decline of cell-to-cell communication in transformed and cancer cells. We also observed a differential degree of communication between two heterotypic cell populations depending on the direction of dye transfer.
