Acupuncture inhibits microglial activation and inflammatory events in the MPTP-induced mouse model
Acupuncture inhibits microglial activation and inflammatory events in the MPTP-induced mouse model
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DOI：
10.1016/j.brainres.2006.10.089
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发表时间：
2007-02-02
期刊：
影响因子：
2.9
通讯作者：
Lim, Sabina
中科院分区：
文献类型：
--
作者：
Kang, Jun Mo;Park, Hi Joon;Lim, Sabina
采用1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)诱导的帕金森病(PD)小鼠模型，观察针刺对小胶质细胞活化和炎症反应的影响，探讨针刺对帕金森病的神经保护作用。给C57BL/6小鼠灌胃MPTP(30 mg/kg)。连续5天。在第一次注射MPTP后2小时开始针刺阳陵泉和太冲穴，然后每隔48小时针刺一次，直到最后一次注射MPTP后1、3和7天处死小鼠进行分析。结果表明，在注射MPTP后第1、3、7天，针刺可抑制纹状体(ST)和黑质(SN)酪氨酸羟化酶(TH)免疫反应性(IR)的下降，并产生神经保护作用。针刺可明显抑制小胶质细胞活化的标志物--巨噬细胞抗原复合体-1(MAC-1)在第1、3、7天的表达，抑制环氧合酶-2(COX-2)和诱导型一氧化氮合酶(INOS)在第1、3、7天的表达。MPTP组第7天纹状体多巴胺(DA)含量为46%，针刺组DA为78%。根据这些结果，我们认为针刺可以作为一种神经保护干预措施，目的是抑制帕金森病小胶质细胞的激活和炎症事件。(C)2006爱思唯尔B.V.保留所有权利。
Using a mouse model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD), this study investigated on the neuroprotective effects of acupuncture by examining whether acupuncture contributed to inhibiting microglial activation and inflammatory events. C57BL/6 mice were treated with MPTP (30 mg/kg, i.p.) for 5 consecutive days. Acupuncture was then applied to acupoints Yanglingquan (GB34) and Taichong (LR3) starting 2 h after the first MPTP administration and then at 48 h intervals until the mice were sacrificed for analyses at 1, 3, and 7 days after the last MPTP injection. These experiments demonstrated that acupuncture inhibited the decreased of the tyrosine hydroxylase (TH) immunoreactivity (IR) and generated a neuroprotective effects in the striatum (ST) and the substantia nigra (SN) on days 1, 3, and 7 post-MPTP injections. Acupuncture attenuated the increase of macrophage antigen complex-1 (MAC-1), a marker of microglial activation, at 1 and 3 days and reduced the increases in cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression on days 1, 3, and 7. In MPTP group, striatal dopamine (DA) was measured by 46% at 7 days, whereas DA in the acupuncture group was 78%. On the basis of these results, we suggest that acupuncture could be used as a neuroprotective intervention for the purpose of inhibiting microglial activation and inflammatory events in PD. (c) 2006 Elsevier B.V. All rights reserved.