Firing responses mediated via distinct nicotinic acetylcholine receptor subtypes in rat prepositus hypoglossi nuclei neurons
Firing responses mediated via distinct nicotinic acetylcholine receptor subtypes in rat prepositus hypoglossi nuclei neurons
复制标题
大鼠舌下核神经元不同烟碱乙酰胆碱受体亚型介导的放电反应
DOI：
10.1152/jn.00168.2018
复制
发表时间：
2018
影响因子：
2.5
通讯作者：
Saito Yasuhiko
中科院分区：
文献类型：
--
作者：
Zhang Yue;Yanagawa Yuchio;Saito Yasuhiko
我们之前报道过，参与凝视控制的舌前核（PHN）中的烟碱乙酰胆碱（ACh）受体（nAChR）介导的胆碱能电流反应可以根据不同的动力学分为不同的类型，并且主要由α7和/或非α7亚型组成：快（F）型、慢速（S）型和快慢（FS）型电流。在本研究中，为了阐明每种电流类型与神经元活动的关系，我们研究了电流类型与膜特性以及每种电流类型引起的放电反应之间的关系。表现出不同后超极化 (AHP) 分布和放电模式的神经元中电流类型的比例不同，这表明 PHN 神经元根据膜特性表现出对特定电流类型的偏好。在对 ACh 的反应中，F 型神经元表现出一种动作电位 (AP) 或多个具有短放电持续时间的 AP，而 S 型神经元则表现出多个具有长放电持续时间的 AP。 F型神经元的放电频率显着高于S型和FS型神经元。 α7亚型特异性拮抗剂消除了F型神经元的放电反应并降低了FS型神经元的反应，但对S型神经元的反应几乎没有影响，而S型神经元的反应被非α7亚型特异性拮抗剂降低。这些结果表明，电流类型的不同特性以及具有不同膜特性的 PHN 神经元中 nAChR 亚型的不同表达，通过 nAChR 的激活产生独特的放电反应。新的和值得注意的舌前核 (PHN) 神经元表现出不同的烟碱乙酰胆碱受体 (nAChR) 介导的电流反应。当前类型的比例在表现出不同后超极化分布和放电模式的神经元中有所不同。具有不同动力学的 nAChR 介导的电流诱导神经元的放电反应，其放电频率和持续时间不同。这些结果表明，具有不同膜特性的 PHN 神经元中当前类型的不同特性通过 nAChR 的激活产生独特的放电反应。
We previously reported that cholinergic current responses mediated via nicotinic acetylcholine (ACh) receptors (nAChRs) in the prepositus hypoglossi nucleus (PHN), which participates in gaze control, can be classified into distinct types based on different kinetics and are mainly composed of α7- and/or non-α7-subtypes: fast (F)-, slow (S)-, and fast and slow (FS)-type currents. In this study, to clarify how each current type is related to neuronal activities, we investigated the relationship between the current types and the membrane properties and the firing responses that were induced by each current type. The proportion of the current types differed in neurons that exhibited different afterhyperpolarization (AHP) profiles and firing patterns, suggesting that PHN neurons show a preference for specific current types dependent on the membrane properties. In response to ACh, F-type neurons showed either one action potential (AP) or multiple APs with a short firing duration, and S-type neurons showed multiple APs with a long firing duration. The firing frequency of F-type neurons was significantly higher than that of S-type and FS-type neurons. An α7-subtype-specific antagonist abolished the firing responses of F-type neurons and reduced the responses of FS-type neurons but had little effect on the responses of S-type neurons, which were reduced by a non-α7-subtype-specific antagonist. These results suggest that the different properties of the current types and the distinct expression of the nAChR subtypes in PHN neurons with different membrane properties produce unique firing responses via the activation of nAChRs.NEW & NOTEWORTHYPrepositus hypoglossi nucleus (PHN) neurons show distinct nicotinic acetylcholine receptor (nAChR)-mediated current responses. The proportion of the current types differed in the neurons that exhibited different afterhyperpolarization profiles and firing patterns. The nAChR-mediated currents with different kinetics induced firing responses of the neurons that were distinct in the firing frequency and duration. These results suggest that the different properties of the current types in PHN neurons with different membrane properties produce unique firing responses via the activation of nAChRs.