Enhanced Fenton Reaction for Xenobiotic Compounds and Lignin Degradation Fueled by Quinone Redox Cycling by Lytic Polysaccharide Monooxygenases
Enhanced Fenton Reaction for Xenobiotic Compounds and Lignin Degradation Fueled by Quinone Redox Cycling by Lytic Polysaccharide Monooxygenases
复制标题
裂解多糖单加氧酶促进醌氧化还原循环促进外源化合物和木质素降解的增强芬顿反应
DOI：
10.1021/acs.jafc.1c01684
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发表时间：
2021-06-15
影响因子：
6.1
通讯作者：
Yu, Hongbo
中科院分区：
文献类型：
--
作者：
Li, Fei;Zhao, Honglu;Yu, Hongbo
Fenton反应被认为在木质纤维素在木材腐烂真菌中的最初攻击中具有重要意义。在真菌中，苯醌氧化还原循环是诱导Fenton反应的主要途径。我们发现，裂解多糖单加氧酶(LPMO)通过LPMO催化的对苯二酚的氧化，可以有效地与葡萄糖脱氢酶(GDH)协同作用，实现对苯二酚的氧化还原循环。LPMO/GDH体系可以增强Fe3+还原活性、H_2O_2的产生和羟基自由基的产生，从而促进Fenton反应。体系产生的羟基自由基对不同的合成染料具有很强的脱色能力，并能降解木质素。我们的结果揭示了LPMOS和Fenton反应之间潜在的关键联系，表明LPMOS可能参与了真菌中的外源化合物和木质素的降解。LPMO的这一新作用可以在生物精炼中开发应用。
The Fenton reaction is considered to be of great significance in the initial attack of lignocellulose in wood-decaying fungi. Quinone redox cycling is the main way to induce the Fenton reaction in fungi. We show that lytic polysaccharide monooxygenases (LPMOs), through LPMO-catalyzed oxidation of hydroquinone, can efficiently cooperate with glucose dehydrogenase (GDH) to achieve quinone redox cycling. The LPMO/GDH system can enhance Fe3+-treducing activity, H2O2 production, and hydroxyl radical generation, resulting in a fueled Fenton reaction. The system-generated hydroxyl radicals exhibited a strong capacity to decolorize different synthetic dyes and degrade lignin. Our results reveal a potentially critical connection between LPMOs and the Fenton reaction, suggesting that LPMOs could be involved in xenobiotic compound and lignin degradation in fungi. This new role of LPMOs may be exploited for application in biorefineries.