Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis)
Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis)
复制标题
半滑舌鳎 (Cynoglossussemilaevis) Sox9a 和 Foxl2 基因的分子克隆、表征和表达分析
DOI：
10.1007/s13131-011-0092-9
复制
发表时间：
2011-01-01
影响因子：
1.4
通讯作者：
Shao Changwei
中科院分区：
文献类型：
--
作者：
Dong Xiaoli;Chen Songlin;Shao Changwei
从半光滑舌底（Cynoglossus semilaevis）中克隆了两个转录因子Sox9a和Foxl2。Sox9a是半乳弧菌Sox9基因的一个新的重复序列。Sox9a基因全长1 842 bp，编码487个氨基酸，Foxl2基因全长1 817 bp，编码308个氨基酸。Sox9a在男性脑、垂体和性腺中表达较高，Foxl2在女性脑、垂体和性腺中表达较高。Sox9a基因在新生雄性性腺中的表达高于正常雌性。Sox9a和Foxl2在原肠期的表达量高于其他时期。在性别分化时期，Sox9a的表达先上升后下降，Foxl2在37 dph时表达较高。Sox9a和Foxl2基因分别在9月龄和12月龄的性腺组织中表达量最高。Sox9a基因被认为与胚胎的性别逆转、性别分化和细胞分化以及生精细胞的形成有着必然的联系。Foxl2被认为在胚胎的性别分化和细胞分化中起作用，但不是性别决定和性别逆转所必需的。
Two transcription factors, Sox9a and Foxl2 were cloned from half-smooth tongue sole (Cynoglossus semilaevis). Sox9a is a new duplication of C.semilaevis Sox9 gene. The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2 were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic cells. Foxl2 was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.