Overexpression of TEX101, a potential novel cancer marker, in head and neck squamous cell carcinoma
Overexpression of TEX101, a potential novel cancer marker, in head and neck squamous cell carcinoma
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DOI：
10.3233/cbm-130302
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Araki, Yoshihiko
中科院分区：
文献类型：
--
作者：
Yoshitake, Hiroshi;Yokoi, Hidenori;Araki, Yoshihiko
TEX101是我们先前发现的Ly-6/尿激酶型纤溶酶原激活剂受体(LU)家族的成员，是一种生殖细胞标志性糖蛋白。到目前为止，据报道，该蛋白家族的一些成员在各种癌症组织中过表达。我们先前报道了LU家族成员Ly6k是小鼠雄性生殖细胞中与TEX101相关的分子。LY6K(人类Ly6K的同源物)在头颈部鳞状细胞癌(HNSCC)中过表达。这些事实使我们推测TEX101可能也存在于HNSCC中，就像LY6K一样。应用已建立的抗人TEX101多克隆抗体，用免疫组织化学方法检测了TEX101蛋白在癌组织中的表达。在HNSCC患者的某些组织标本中检测到TEX101，而在正常鳞状上皮细胞中检测到TEX101。喉癌组织中有54例(80.6%)癌细胞表达TEX101蛋白。与TEX101弱阳性和阴性患者相比，TEX101阳性患者的淋巴结转移率低。本研究结果提示TEX101是一个新的肿瘤相关蛋白，可作为判断HNSCC预后/诊断的指标。
TEX101, a member of the Ly-6/urokinase-type plasminogen activator receptor (LU)-family we previously identified, is a germ cell-marker glycoprotein. To date, it is reported that some members of the protein-family are overexpressed in a variety of cancer tissues. We previously reported Ly6k, a member of the LU-family, as an association molecule with TEX101 in murine male germ cells. LY6K (a human homologue of Ly6k) is overexpressed in head and neck squamous cell carcinomas (HNSCC). These facts led us to speculate that TEX101 may also exist in HNSCC, like LY6K. Using an anti-human TEX101 polyclonal antibody (pAb) established, we examined the expression of TEX101 protein in cancer tissues by immunohistochemical analysis. TEX101 was detected in the cancer cells of some tissue specimens from patients with HNSCC, whereas the normal squamous epithelium was immunonegative. The TEX101 protein was detected in cancer cells from 54 out of 64 (80.6%) patients with HNSCC. The rate of lymph nodes metastasis tends to be low in TEX101-positive patients, compared to patients with weakly positive and negative expression of TEX101. The present results imply that TEX101 is a novel cancer-related protein and may be useful as a marker for prognosis/diagnosis of HNSCC.