A SURVEY OF ISOZYME ANALYSIS OF WILD MANDARIN, TACHIBANA (CITRUS-TACHIBANA (MAK) TANAKA) GROWING IN JAPAN
A SURVEY OF ISOZYME ANALYSIS OF WILD MANDARIN, TACHIBANA (CITRUS-TACHIBANA (MAK) TANAKA) GROWING IN JAPAN
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DOI：
10.2503/jjshs.59.1
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发表时间：
1990-06-01
影响因子：
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通讯作者：
KAJIURA, I
中科院分区：
文献类型：
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作者：
HIRAI, M;MITSUE, S;KAJIURA, I
Tchibana(Citrus Tchibana(Mak.)Tanaka)的同工酶基因进行了调查和分析。从静冈县的伊豆半岛到宫崎县的石南，都观察到了许多树木。虽然它们中的大多数是栽培的，但也有一些是野生的。同工酶分析表明，从中国引进的红花品种与红花品种之间存在明显的差异。在Px(过氧化物酶)基因座上，几乎所有的立花树都具有CC基因型，而大多数中国柑橘在Px(过氧化物酶)座位上都有DD基因。此外，谷草转氨酶(GOT)-2上的等位基因A是立竹种群所特有的。在红花种群的一些个体中发现了GOT-3上罕见的等位基因T，这在其他柑橘中都没有记录到。在立花种群中也观察到同工酶基因的异质性。这些发现表明，在亚洲大陆的其他柑橘中，已经从基因上分离出了红花，并且是原产于日本的。在红花种植区发现了几个红花与栽培的中国橘子的天然杂交品种。
Tachibana (Citrus tachibana (Mak.) Tanaka) trees growing in the wild and cultivated in Japan were surveyed and analyzed for their isozyme genes. Many trees were observed in south-western areas from Izu Peninsula, Shizuoka Prefecture to Ishinami, Kushima, Miyazaki Prefecture. Although most of them are cultivated, some are suggested to be wild. Isozyme analysis of the tachibana population clearly demonstrated a difference between the tachibana and manadarin cultivars introduced from China. Almost all tachibana trees examined had CC genotype at Px (peroxidase) locus, while most of the Chinese mandarins are reported to have DD at Px. In addition, allele A at Got (glutamate oxalacetate transaminase)-2 was unique to the tachibana population. A rare allele T at Got-3, which has not been recorded in any other citrus, was found in some individuals of the tachibana population. A heterogeneity for isozyme genes was also observed in the tachibana population. These findings indicate that tachibana has been genetically isolated from other citrus in the Asian Continent, and is native to Japan. Several natural hybrids between tachibana and cultivated Chinese mandarins were found in the tachibana-growing areas.