Astaxanthin inhibits reactive oxygen species-mediated cellular toxicity in dopaminergic SH-SY5Y cells via mitochondria-targeted protective mechanism
Astaxanthin inhibits reactive oxygen species-mediated cellular toxicity in dopaminergic SH-SY5Y cells via mitochondria-targeted protective mechanism
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DOI：
10.1016/j.brainres.2008.11.076
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发表时间：
2009-02-13
期刊：
影响因子：
2.9
通讯作者：
Osawa, Toshihiko
中科院分区：
文献类型：
--
作者：
Liu, Xuebo;Shibata, Takahiro;Osawa, Toshihiko
虾青素是一种强大的抗氧化剂，天然存在于各种生物体中。本研究旨在探讨虾青素对活性氧（reactive oxygen species，ROS）介导的多巴胺能SH-SY 5 Y细胞凋亡的影响及其机制。用DHA氢过氧化物（DHA-OOH）或6-羟基多巴胺（6-OHDA）处理，其中任一种都是ROS诱导的神经毒素，通过MTT测定导致存活的多巴胺能SH-SY 5 Y细胞显著减少，而用虾青素预处理细胞时显示出显著的保护作用。此外，100 nM虾青素预处理显着抑制细胞凋亡，线粒体异常和细胞内ROS的产生发生在DHA-OOH或6-OHDA处理的细胞。虾青素的神经保护作用被认为是依赖于其抗氧化潜力和线粒体保护，因此，它建议，虾青素可能是一种有效的治疗氧化应激相关的神经变性。(C)2008 Elsevier B. V.保留所有权利。
Astaxanthin is a powerful antioxidant that occurs naturally in a wide variety of living organisms. The aim of this study is to investigate the effect and the mechanism of astaxanthin on reactive oxygen species (ROS)-mediated apoptosis in dopaminergic SH-SY5Y cells. The treatment with DHA hydroperoxide (DHA-OOH) or 6-hydroxydopamine (6-OHDA), either of which is ROS-inducing neurotoxin, led to a significant decrease in viable dopaminergic SH-SY5Y cells by MTT assay, whereas a significant protection was shown while the cells were pretreated with astaxanthin. Moreover, 100 nM astaxanthin pretreatment significantly inhibited apoptosis, mitochondrial abnormalities and intracellular ROS generation occurred in either DHA-OOH- or 6-OHDA-treated cells. The neuroprotective effect of astaxanthin is suggested to be dependent upon its antioxidant potential and mitochondria protection; therefore, it is suggested that astaxanthin may be an effective treatment for oxidative stress-associated neurodegeneration. (C) 2008 Elsevier B.V. All rights reserved.