Development of a novel method for measuring covalent binding and its application to investigations of bromobenzene hepatotoxicity.
Development of a novel method for measuring covalent binding and its application to investigations of bromobenzene hepatotoxicity.
复制标题
开发一种测量共价结合的新方法及其在溴苯肝毒性研究中的应用。
DOI：
10.1007/978-1-4757-0674-1_16
复制
发表时间：
1981
影响因子：
--
通讯作者：
J. D. Sun
中科院分区：
文献类型：
--
作者：
J. Dent;J. D. Sun
化学物质或代谢物与细胞组织大分子的共价结合可能代表化学毒性的重要机制。共价相互作用被认为与肝、肾、肺和骨髓毒性以及致癌性有关（Mitchell 等，1976；Mitchell 等，1977；Hinson，1980；Gillette 等，1974；Boyd，1980；Irons 等，1980；Miller，1970）。最近，很明显，在生物系统中测量的共价结合量可能不是与毒理学最相关的参数。 Gillette (1974) 强调，反应性代谢物可能与非关键大分子结合，并且这种相互作用可能很少或没有功能意义。为了进一步了解共价结合在化学毒性中的作用，有必要研究共价结合的特异性。
The covalent binding of chemicals or metabolites to cellular tissue macromolecules may represent an important mechanism for chemical toxicity. Covalent interactions have been suggested to be involved in hepatic, renal, pulmonary and bone marrow toxicity as well as carcinogenicity (Mitchell et al., 1976; Mitchell et al., 1977; Hinson, 1980; Gillette et al., 1974; Boyd, 1980; Irons et al., 1980; Miller, 1970). More recently it has become apparent that the amount of covalent binding measured in a biological system may not be the most toxicologically relevant parameter. Gillette (1974) emphasized that reactive metabolites may bind to noncritical macromolecules and such interaction may be of little or no functional significance. To further our understanding of the role of covalent binding in chemical toxicity it is necessary to study the specificity of covalent binding.