Carbohydrate metabolism in contracting dog skeletal muscle in situ.
Carbohydrate metabolism in contracting dog skeletal muscle in situ.
复制标题
原位收缩狗骨骼肌中的碳水化合物代谢。
DOI：
10.1152/ajplegacy.1968.215.5.995
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
W. Stainsby
中科院分区：
文献类型：
--
作者：
C. Chapler;W. Stainsby
方法实验在杂种犬上进行。在实验室使用前，所有动物均进行了去甲处理，并隔离至少2周。在此期间，它们每天都被随意喂食，商业（Purina）狗饲料。在实验的早晨，他们没有被喂食。通过静脉注射戊巴比妥钠30 mg/kg麻醉动物，并在需要时给予额外剂量30 mg。在用于糖原研究的动物中，通过从腘区延伸至踝部的内侧切口，手术暴露两后肢中的腓肠肌-跖肌群。覆盖在每个肌肉群上的结缔组织和脂肪被小心地分离和收缩。暴露的肌肉用盐水浸泡的纱布覆盖，以防止蒸发干燥和冷却。在实验期之前的手术暴露使得可以在实验完成后的几秒钟内移除肌肉，以避免糖原分解。
METHODSAU of the experiments were performed on mongrel dogs. Prior to use in the laboratory, all of the animals were deparisitized and kept in quarantine for at least 2 weeks. During this period they were fed daily, ad lib., commercial(Purina) dog chow. They were not fed the morning of the experiment. The animals were anesthetized with an intravenous injection of sodium pentobarbital, 30 mg/kg, and additional doses of 30 mg were administered when needed.Procedures for Ute glycogen study. In animals used for the glycogen study, the gastrocnemius-plantaris muscle group in both hindlimbs was surgically exposed through medial incisions extending from the popliteal region to the ankle. The connective tissue and fat which covered each muscle group were carefully separated and retracted. The exposed muscles were covered with salinesoaked gauze to prevent evaporative drying and cooling. The surgical exposure, prior to the experimental period, made it possible to remove the muscles within a few seconds after the experiment was completed in an effort to avoid glycogenolysis.