A physiological role for gene loops in yeast
A physiological role for gene loops in yeast
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DOI：
10.1101/gad.1823609
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发表时间：
2009-11-15
影响因子：
10.5
通讯作者：
Hampsey, Michael
中科院分区：
文献类型：
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作者：
Laine, Jean-Philippe;Singh, Badri Nath;Hampsey, Michael
在酵母和哺乳动物细胞中已鉴定出能使RNA聚合酶II转录基因的启动子和终止子区域并列的DNA环。环的形成依赖转录，并需要前体mRNA 3'端加工机制的组分。在此我们报道，在酵母GAL10基因处的环在一个转录激活和抑制周期之后仍然存在。此外，GAL10以及一个GAL1p - SEN1报告基因在一个激活和抑制周期之后呈现快速的再激活动力学——一种被定义为“转录记忆”的现象——并且这种效应与环的持续存在相关。我们提出基因环促进酵母中的转录记忆。
DNA loops that juxtapose the promoter and terminator regions of RNA polymerase II-transcribed genes have been identified in yeast and mammalian cells. Loop formation is transcription-dependent and requires components of the pre-mRNA 3'-end processing machinery. Here we report that looping at the yeast GAL10 gene persists following a cycle of transcriptional activation and repression. Moreover, GAL10 and a GAL1p-SEN1 reporter undergo rapid reactivation kinetics following a cycle of activation and repression-a phenomenon defined as "transcriptional memory''-and this effect correlates with the persistence of looping. We propose that gene loops facilitate transcriptional memory in yeast.