Rhodococcus sp Q5, a novel agarolytic bacterium isolated from printing and dyeing wastewater
Rhodococcus sp Q5, a novel agarolytic bacterium isolated from printing and dyeing wastewater
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红球菌属
DOI：
10.1007/s12223-012-0150-5
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发表时间：
2012-09-01
影响因子：
2.6
通讯作者：
Li, Mengying
中科院分区：
文献类型：
--
作者：
Feng, Zehua;Peng, Lin;Li, Mengying
一种琼脂降解细菌，红球菌属。使用含有琼脂作为唯一碳源的矿物盐琼脂平板从印染废水中分离出Q5。该细菌在 pH 4.0 至 9.0、15 至 35A 摄氏度以及 0-5% NaCl 浓度下生长；最佳值为 pH 6.0、30A 摄氏度和 1% NaCl。在 pH 6.0 和 30A 摄氏度下观察到最大琼脂酶产量。该细菌不需要 NaCl 来生长或产生琼脂酶。 Q5 分泌的琼脂酶可被琼脂诱导，并被除乳糖之外的所有测试单糖抑制。菌株Q5可以水解淀粉，但不能水解纤维素或羧甲基纤维素。当乳糖或淀粉是碳和能量的唯一来源时，也可以在培养基中检测到琼脂酶活性。菌株Q5可以在无氮矿物培养基中生长；有机氮源比无机碳源对于生长和琼脂酶生产更有效。向培养基中添加更多的有机氮（蛋白胨）对应于琼胶酶活性的降低。
An agar-degrading bacterium, Rhodococcus sp. Q5, was isolated from printing and dyeing wastewater using a mineral salts agar plate containing agar as the sole carbon source. The bacterium grew from pH 4.0 to 9.0, from 15 to 35A degrees C, and in NaCl concentrations of 0-5 %; optimal values were pH 6.0, 30A degrees C, and 1 % NaCl. Maximal agarase production was observed at pH 6.0 and 30A degrees C. The bacterium did not require NaCl for growth or agarase production. The agarase secreted by Q5 was inducible by agar and was repressed by all simple sugars tested except lactose. Strain Q5 could hydrolyze starch but not cellulose or carboxymethyl cellulose. Agarase activity could also be detected in the medium when lactose or starch was the sole source of carbon and energy. Strain Q5 could grow in nitrogen-free mineral media; an organic nitrogen source was more effective than inorganic carbon sources for growth and agarase production. Addition of more organic nitrogen (peptone) to the medium corresponded with reduced agarase activity.