Static magnetic fields promote osteoblast-like cells differentiation via increasing the membrane rigidity
Static magnetic fields promote osteoblast-like cells differentiation via increasing the membrane rigidity
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DOI：
10.1007/s10439-007-9370-2
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Huang, Haw-Ming
中科院分区：
文献类型：
--
作者：
Chiu, Kang-Hsuan;Ou, Keng-Liang;Huang, Haw-Ming
本研究的目的是测试成骨细胞的分化作用后，与静态磁场（SMF）处理。MG 63成骨细胞样细胞暴露于0.4-T SMF。通过观察碱性磷酸酶活性的变化和电镜图像来评估分化标志物。膜流动性用于评估SMF模拟后细胞膜的生物物理性质的改变。我们的研究结果表明，SMF暴露增加碱性磷酸酶活性和细胞外基质释放MG 63细胞。另一方面，暴露于0.4-T SMF的MG 63细胞在6 h时表现出荧光各向异性显著增加，在24 h时观察到生长因子的增殖效应显著降低。基于这些发现，作者认为SMF影响成骨细胞成熟的可能机制之一是通过增加膜刚度和减少生长因子在膜结构域的增殖促进作用。
The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Membrane fluidity was used to evaluate alterations in the biophysical properties of the cellular membranes after the SMF simulation. Our results show that SMF exposure increases alkaline phosphatase activity and extracellular matrix release in MG63 cells. On the other hand, MG63 cells exposed to a 0.4-T SMF exhibited a significant increase in fluorescence anisotropy at 6 h, with a significant reduction in the proliferation effects of growth factors noted at 24 h. Based on these findings, the authors suggest that one of the possible mechanisms that SMF affects osteoblastic maturation is by increasing the membrane rigidity and reducing the proliferation-promoting effects of growth factors at the membrane domain.