Expression of the atrial-specific myosin heavy chain AMHC1 and the establishment of anteroposterior polarity in the developing chicken heart.
Expression of the atrial-specific myosin heavy chain AMHC1 and the establishment of anteroposterior polarity in the developing chicken heart.
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DOI：
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发表时间：
1994-04
期刊：
影响因子：
4.6
通讯作者：
K. Yutzey;J. Rhee;D. Bader
中科院分区：
文献类型：
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作者：
K. Yutzey;J. Rhee;D. Bader
分离出一种独特的肌球蛋白重链 cDNA (AMHC1)，仅在发育中的鸡心脏的心房中表达，并用于研究多样化心肌细胞谱系的生成。通过整体原位杂交确定心脏形成过程中 AMHC1 基因的表达模式。当这些肌细胞最初分化时（Hamburger 和 Hamilton 阶段 9+），AMHC1 首先在心脏后段被激活。尽管心室肌球蛋白重链亚型从 8+ 阶段开始强烈表达，但在此阶段心脏的前段不表达 AMHC1。在鸡的整个发育过程中，AMHC1 在后心管发育成多样化的心房时持续表达。后心肌细胞中 AMHC1 表达的早期激活表明，心脏细胞在最初分化时是多样化的，并且前心脏祖细胞与后心脏祖细胞的肌球蛋白同种型基因表达不同。通过用视黄酸作为心脏原基融合体处理胚胎，AMHC1 的表达域可以在心管内向前扩展。在心脏原基融合开始之前用视黄酸处理的胚胎在整个心脏形成区域表达AMHC1，并且心脏原基的融合受到抑制。这些数据表明，视黄酸治疗会导致心脏后部（心房）区域的扩张，并表明心肌祖细胞的多样化命运可以被改变。
A unique myosin heavy chain cDNA (AMHC1), which is expressed exclusively in the atria of the developing chicken heart, was isolated and used to study the generation of diversified cardiac myocyte cell lineages. The pattern of AMHC1 gene expression during heart formation was determined by whole-mount in situ hybridization. AMHC1 is first activated in the posterior segment of the heart when these myocytes initially differentiate (Hamburger and Hamilton stage 9+). The anterior segment of the heart at this stage does not express AMHC1 although the ventricular myosin heavy chain isoform is strongly expressed beginning at stage 8+. Throughout chicken development, AMHC1 continues to be expressed in the posterior heart tube as it develops into the diversified atria. The early activation of AMHC1 expression in the posterior cardiac myocytes suggests that the heart cells are diversified when they differentiate initially and that the anterior heart progenitors differ from the posterior heart progenitors in their myosin isoform gene expression. The expression domain of AMHC1 can be expanded anteriorly within the heart tube by treating embryos with retinoic acid as the heart primordia fuse. Embryos treated with retinoic acid prior to the initiation of fusion of the heart primordia express AMHC1 throughout the entire heart-forming region and fusion of the heart primordia is inhibited. These data indicate that retinoic acid treatment produces an expansion of the posterior (atrial) domain of the heart and suggests that diversified fates of cardiomyogenic progenitors can be altered.