Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1:: MexCD-OprJ is an inducible pump
Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1:: MexCD-OprJ is an inducible pump
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DOI：
10.1111/j.1574-6968.2001.tb10794.x
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发表时间：
2001-08-07
影响因子：
2.1
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
Morita, Y;Komori, Y;Tsuchiya, T
我们构建了一系列缺失突变体，缺失Mex多药外排泵的所有四个主要Mex操纵子或拥有铜绿假单胞菌PAO 1的每一个操纵子。研究了MexAB-OprM、MexXY-OprM和MexCD-OprJ的药物特异性。令人惊讶的是，我们发现MexCD-OprJ是一种诱导型泵，其诱导剂是氯化四苯基鏻、溴化乙锭、罗丹明6 G和吖啶黄。氟喹诺酮类、氯霉素、红霉素和四环素虽然是MexCD-OprJ的底物，但不是诱导剂。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.