The effect of myosin antibody on the division of starfish blastomeres.
The effect of myosin antibody on the division of starfish blastomeres.
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DOI：
10.1083/jcb.74.1.251
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发表时间：
1977-07
影响因子：
7.8
通讯作者：
Okuno, M
中科院分区：
文献类型：
--
作者：
Mabuchi, I;Okuno, M
制备了海星卵肌球蛋白抗血清。通过Ouchterlony免疫扩散试验和十二烷基硫酸钠（SDS）存在下的免疫电泳证明了抗体对肌球蛋白的特异性。后一种技术表明，抗体结合鸡蛋肌球蛋白的重链和轻链。此外，抗体与海星精子肌球蛋白和海星成体肌球蛋白的重链和轻链均发生反应。在Ouchterlony试验中，它不与牛血小板肌球蛋白或兔骨骼肌肌球蛋白反应;然而，在SDS存在下，在抗体与这些肌球蛋白重链之间观察到弱反应。该抗体对卵肌球蛋白Ca-和Mg-ATPase活性无影响，但对肌动蛋白激活的ATPase活性有抑制作用。将抗体显微注射到海星卵的二细胞期卵裂球中。当在间期注射抗卵肌球蛋白γ-球蛋白时，超过0.3ng的量抑制随后的裂解。当注射在接近裂解开始时进行时，抑制降低。在第二次切割开始时，抗体不具有抑制性;然而，适量抑制第三次切割。尽管在抗体存在下观察到核膜消失，但有丝分裂器的形成或多或少受到干扰。然而，子核的形成似乎几乎不受抗体的影响，除了核之间的距离显着小于正常。
Antiserum against starfish egg myosin was produced in rabbits. Antibody specificity to myosin was demonstrated by Ouchterlony's immunodiffusion test and by immunoelectrophoresis in the presence of sodium dodecylsulfate (SDS). The latter technique showed that the antibody binds to both heavy and light chains of egg myosin. Furthermore, the antibody reacted with starfish sperm mysosin and starfish adult muscle myosin at both the heavy and light chains. It did not react with bovine platelet mysosin or rabbit skeletal muscle myosin in Ouchterlony's test; however, a weak reaction was observed in the presence of SDS between the antibody and these myosin heavy chains. Ca- and Mg-ATPase activities of egg myosin were not affected by the antibody, but it did inhibit actin-activated ATPase activity of egg myosin. Microinjection of the antibody into blastomeres of starfish eggs at the two-cell stage was carried out. Anti-egg myosin γ-globulin inhibited the subsequent cleavages at an amount of more than 0.3 ng when injected at interphase. The inhibition was reduced when the injection was carried out near the initiation of cleavage. At the onset of the second cleavage the antibody was not inhibitory; however, an appropriate amount inhibited the third cleavage. Although the disappearance of the nuclear membrane was observed in the presence of the antibody, the formation of the mitotic apparatus was more or less disturbed. However the formation of daughter nuclei seemed to be scarcely affected by the antibody except that the distance between the nuclei was significantly smaller than normal.