Characterization of VHε gene expressed in PBL from children with atopic diseases:: detection of homologous VH1-69 derived transcripts from three unrelated patients
Characterization of VHε gene expressed in PBL from children with atopic diseases:: detection of homologous VH1-69 derived transcripts from three unrelated patients
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DOI：
10.1016/j.imlet.2004.04.006
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发表时间：
2004-06-15
影响因子：
4.4
通讯作者：
Ra, C
中科院分区：
文献类型：
--
作者：
Bando, Y;Shimizu, A;Ra, C
为了阐明婴儿早期IgE产生的分子背景，我们分析了3例过敏性疾病婴儿PBL中表达的36个V-H-Cyclic转录本的核苷酸序列。我们检测了来自V（H）1、V（H）3、V（H）4基因家族成员的转录本，在特定V-H基因家族的使用中没有观察到偏倚。然而，一些V-H成员，V（H）1; 1-46，1-69，V（H）3; 3-11，3-21，V（H）4; 4-39，4-59经常被看到，因此值得注意。1例重度特应性皮炎伴食物过敏患者VH 4基因为显性，提示该基因参与了该病的发病。即使分析了有限数量的克隆，我们也发现了来自所有三名患者的高度同源的VH 1-69衍生序列，其在互补决定区（CDR）1和2中具有相同的体细胞突变或多态性变异，具有相同的CDR 3（D-J（H））序列，包括接头。这些发现可能表明，一个相当有限的VH基因可能会重新排列的特异性IgE在生命早期。(C)2004 Elsevier B. V.保留所有权利。
To elucidate the molecular back-ground of IgE production in early infancy, we analyzed the nucleotide sequences of 36 V-H-Cepsilon transcripts expressed in PBL from three infants with allergic diseases. We detected transcripts derived from V(H)1, V(H)3, V(H)4 gene family members, and no bias was observed in the usage of particular V-H gene family. However, some V-H members, V(H)1; 1-46, 1-69, V(H)3; 3-11, 3-21, V(H)4; 4-39, 4-59 were frequently seen and thus notable. VH4 gene was dominant in one patient with severe atopic dermatitis and food allergy, suggesting the involvement of this gene in pathogenesis of the disease. Even a limited number of clones were analyzed, we also found highly homologous VH 1-69 derived sequences from all the three patients, which share the same somatic mutations or polymorphic variations in complementarity-determining region (CDR) 1, and 2 with the same CDR3 (D-J(H)) sequences including the junctions. These findings might suggest that a rather limited VH gene might be rearranged for specific IgE in early life. (C) 2004 Elsevier B.V. All rights reserved.