LOCALIZATION OF ACTIN MESSENGER-RNA DURING EARLY ASCIDIAN DEVELOPMENT
LOCALIZATION OF ACTIN MESSENGER-RNA DURING EARLY ASCIDIAN DEVELOPMENT
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DOI：
10.1016/0012-1606(83)90290-7
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
BRODEUR, RD
中科院分区：
文献类型：
--
作者：
JEFFERY, WR;TOMLINSON, CR;BRODEUR, RD
用聚尿苷酸和克隆的DNA探针进行原位杂交，测定了海鞘（Styela plicata）早期发育过程中RNA序列的空间分布。海鞘卵和胚胎含有3个具有特定形态发生命运的有色细胞质区域：外质、内质和肌质。这些细胞质区域参与受精后的卵质分离，并在早期胚胎发生过程中分布到不同的细胞谱系中。与poly（U）的原位杂交表明，poly（A）+ [腺苷酸] RNA在卵和胚胎中分布不均匀，约45%在外质中，50%在内质中，只有5%在肌质中。用组蛋白DNA探针进行的原位杂交表明，组蛋白RNA序列不定位于卵或胚胎中，而是根据体积分布在3个胞质区域之间。用肌动蛋白DNA探针进行的原位杂交表明，肌动蛋白RNA定位于卵和胚胎的肌质和外质中，约45%存在于肌质中，40%存在于外质中，仅15%存在于内质中。大部分的卵肌动蛋白mRNA定位于肌浆中，受精后参与卵浆分离，并在胚胎发生过程中差异分布到中胚层细胞系。卵mRNA的翻译产物的分析表明，本地化的mRNA编码的细胞质肌动蛋白亚型。
Spatial distribution of RNA sequences during early development of the ascidian, Styela plicata, was determined by in-situ hybridization with poly(U) [uridylic acid] and cloned DNA probes. Styela eggs and embryos contain 3 colored cytoplasmic regions of specific morphogenetic fates: the ectoplasm, endoplasm and myoplasm. These cytoplasmic regions participate in ooplasmic segregation after fertilization and are distributed to different cell lineages during early embryogenesis. In-situ hybridization with poly(U) suggests that poly(A)+ [adenylate] RNA is unevenly distributed in eggs and embryos with about 45% in the ectoplasm, 50% in the endoplasm and only 5% in the myoplasm. In-situ hybridization with a histone DNA probe showed that histone RNA sequences were not localized in eggs or embryos and distributed between the 3 cytoplasmic regions according to their volumes. In-situ hybridization with an actin DNA probe showed actin RNA was localized in the myoplasm and ectoplasm of eggs and embryos with about 45% present in the myoplasm, 40% in the ectoplasm and only 15% in the endoplasm. A large proportion of the egg actin mRNA is localized in the myoplasm, participates in ooplasmic segregation after fertilization, and is differentially distributed to the mesodermal cell lineages during embryogenesis. Analysis of the translation products of egg mRNA suggests that the localized mRNA codes for a cytoplasmic actin isoform.