Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells
Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells
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DOI：
10.1016/j.jcv.2007.09.003
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发表时间：
2007-12-01
影响因子：
8.8
通讯作者：
Hayden, R. T.
中科院分区：
文献类型：
--
作者：
Perlman, J.;Gibsonb, C.;Hayden, R. T.
背景资料：检测和定量外周血标本中的腺病毒（ADV）已成为免疫抑制患者管理中越来越重要的工具。研究人员已经描述了使用全血（WB），外周血单核细胞（PBMC），血清和血浆，但没有研究比较了这些不同的样品类型用于在临床诊断assay.Objectives的效用：为了确定最佳的血液室定量实时测量腺病毒在外周血标本。研究设计：采用实时荧光定量PCR（qrt-PCR）检测148例患者的WB、PBMC和血浆共338份标本中ADV，并比较阳性标本中ADV定性敏感性和病毒载量的一致性。WB和血浆的定量值相似，并且倾向于大于PBMC样品中发现的定量值。连续阳性样品在个别患者的比较表明，病毒载量跟踪类似随着时间的推移，无论样品类型tested.Conclusion：虽然WB和血浆不提供显着增加PBMC的灵敏度，他们可以提供的好处，在降低处理成本和实验室周转时间。(C)2007 Elsevier B.V.保留所有权利。
Background: Detection and quantification of adenovirus (ADV) in peripheral blood specimens has become an increasingly important tool in the management of immunosuppressed patients. Investigators have described the use of whole blood (WB), peripheral blood mononuclear cells (PBMC), serum and plasma but no studies have compared the utility of these different sample types for use in a clinical diagnostic assay.Objectives: To determine the optimal blood compartment for quantitative real-time measurement of adenovirus in peripheral blood specimens. Study design: WB, PBMC, and plasma representing 338 samples from 148 patients were tested for ADV by quantitative real-time PCR (qrt-PCR) and the results compared for concordance of both qualitative sensitivity and viral load among positive specimens.Results: There was no significant difference in qualitative sensitivity among the three tested specimen types. Quantitative values of WB and plasma were similar and tended to be greater than those found in PBMC samples. Comparison of consecutive positive samples within individual patients showed that viral loads tracked similarly over time, irrespective of the sample type tested.Conclusion: While WB and plasma do not offer a significant increase in sensitivity over PBMC, they may offer benefits in terms of reduced processing costs and laboratory turn around time. (C) 2007 Elsevier B.V. All rights reserved.