Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes
Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes
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DOI：
10.1007/s12274-015-0736-8
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发表时间：
2015-07-01
期刊：
影响因子：
9.9
通讯作者：
Xi, Peng
中科院分区：
文献类型：
--
作者：
Chen, Xuanze;Zeng, Zhiping;Xi, Peng
使用先前开发的定位超分辨率技术不能容易地实现三维成像。在这里，我们提出了一种三维多模亚衍射成像技术与旋转盘（SD）共聚焦显微镜称为3D-MUSIC，它不仅具有所有的优点，SD共聚焦显微镜，如成像速度快，高信噪比，和光学切片能力，但也扩展了其空间分辨率限制沿着所有的三维。轴向和横向分辨率都可以通过改进的荧光探针的闪烁/波动性质同时提高，例如量子点。此外，具有双模态的超分辨率图像可以通过超分辨率光学波动成像（SOFI）和漂白/闪烁辅助定位显微镜（BaLM）获得。因此，使用SD-SOFI可以通过仅捕获100帧来实现快速超分辨率成像，而SD-BaLM可以产生高分辨率成像。
Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk (SD) confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD confocal microscopy, such as fast imaging speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along all three dimensions. Both axial and lateral resolution can be improved simultaneously by virtue of the blinking/fluctuating nature of modified fluorescent probes, exemplified with the quantum dots. Further, super-resolution images with dual modality can be obtained through super-resolution optical fluctuation imaging (SOFI) and bleaching/blinking-assisted localization microscopy (BaLM). Therefore, fast super-resolution imaging can be achieved with SD-SOFI by capturing only 100 frames while SD-BaLM yields high-resolution imaging.