A simple 3D cryogel co-culture system used to study the role of CAFs in EMT of MDA-MB-231 cells
A simple 3D cryogel co-culture system used to study the role of CAFs in EMT of MDA-MB-231 cells
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DOI：
10.1039/c6ra28721b
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发表时间：
2017-03
期刊：
影响因子：
3.9
通讯作者：
Ge Zhang;Xiaoping Song;Jie Mei;Genlan Ye;Leyu Wang;Lei Yu;M. Xing;Xiaozhong Qiu
中科院分区：
文献类型：
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作者：
Ge Zhang;Xiaoping Song;Jie Mei;Genlan Ye;Leyu Wang;Lei Yu;M. Xing;Xiaozhong Qiu
乳腺癌是最常见的上皮性肿瘤之一，严重危害妇女健康。研究表明，癌相关成纤维细胞（CAFs）具有肌成纤维细胞的特性，在乳腺癌的生长和转移中起重要作用。由于缺乏适用的动物模型，CAFs在肿瘤转移中的确切作用仍不清楚。在这项研究中，一个简单的三维（3D）冷冻凝胶共培养系统的开发，以评估CAFs在乳腺癌转移的作用。结果发现，CAFs可以促进MDA-MB-231细胞（人乳腺癌细胞）在体外三维冷冻凝胶共培养系统中的上皮-间充质转化。然后利用共培养人乳腺癌细胞系统构建异种移植瘤小鼠体内模型。结果发现，CAF可促进MDA-MB-231细胞在体内异种移植肿瘤小鼠模型中的恶化和转移。
Breast cancer is one of the most common epithelial tumors and can seriously endanger women's health. Studies have shown that carcinoma-associated fibroblasts (CAFs) possess myofibroblast features and play an important role in the growth and metastasis of breast cancer. Because of the lack of an applicable animal model, the exact role of CAFs in tumor metastasis is still unclear. In this research, a simple three-dimensional (3D) cryogel co-culture system was developed to evaluate the role of CAFs in breast cancer metastasis. It was found that CAFs could promote epithelial–mesenchymal transition of MDA-MB-231 cells (human breast cancer cells) in a 3D cryogel co-culture system in vitro. Then the co-culture human breast cancer system was used to construct a xenograft tumor mouse model in vivo. It was found that CAFs could promote deterioration and metastasis of MDA-MB-231 cells in a xenograft tumor mouse model in vivo.