Alterations in thigh subcutaneous adipose tissue gene expression in protease inhibitor-based highly active antiretroviral therapy
Alterations in thigh subcutaneous adipose tissue gene expression in protease inhibitor-based highly active antiretroviral therapy
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DOI：
10.1016/j.metabol.2004.08.022
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发表时间：
2005-05-01
影响因子：
9.8
通讯作者：
Li, E
中科院分区：
文献类型：
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作者：
Chaparro, J;Reeds, DN;Li, E
使用基于蛋白酶抑制剂（PI）的高效抗逆转录病毒疗法（HAART）与局部脂肪分布改变、胰岛素抵抗和血脂异常有关。为了评估基于PI的HAART如何影响男性HIV-1感染患者的脂肪细胞基因表达，使用逆转录-聚合酶链反应定量来自男性（1）HIV-1血清阴性受试者的大腿和腹部皮下脂肪组织中脂肪细胞转录因子和脂肪细胞因子的信使RNA表达（对照，n = 9），（2）无症状的未经治疗的HIV-1感染患者（初治，n = 6），（3）接受抗逆转录病毒药物但从未接受PI的HIV-1感染患者（PI初治，n = 5），（4）接受基于PI的HAART治疗的HIV-1感染患者（PI，n = 7），和（5）在入组前超过6个月停止抗病毒治疗的PI组分的HIV-1感染患者（既往PI，n =7）。在PI组，CCAAT/增强子结合蛋白α-瘦素和脂联素的mRNA表达水平（18%，P
Use of protease inhibitor (PI) -based highly active antiretroviral therapy (HAART) has been associated with altered regional fat distribution, insulin resistance, and dyslipidemias. To assess how PI-based HAART affects adipocyte gene expression in male HIV-1-infected patients, reverse transcription-polymerase chain reaction was used to quantify messenger RNA expression of adipocyte transcription factors and adipocytokines in thigh and abdominal subcutaneous adipose tissue from male (1) HIV-1 seronegative subjects (control, n = 9), (2) asymptomatic treatment-naive HIV-1-infected patients (naive, n = 6), (3) HIV-1-infected patients who were receiving antiretroviral agents but never received PIs (PI naive, n = 5), (4) HIV-1-infected patients who were receiving PI-based HAART (PI, n = 7), and (5) HIV-1-infected patients who discontinued the PI component of their antiviral therapy more than 6 months before enrollment (past PI, n =7). In the PI group, the messenger RNA expression levels of the CCAAT/enhancer-binding protein alpha leptin, and adiponectin (18%, P