MCP AND FAB-7 - MOLECULAR ANALYSIS OF PUTATIVE BOUNDARIES OF CIS-REGULATORY DOMAINS IN THE BITHORAX COMPLEX OF DROSOPHILA-MELANOGASTER
MCP AND FAB-7 - MOLECULAR ANALYSIS OF PUTATIVE BOUNDARIES OF CIS-REGULATORY DOMAINS IN THE BITHORAX COMPLEX OF DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1093/nar/22.15.3138
复制
发表时间：
1994-08-11
影响因子：
14.9
通讯作者：
SCHEDL, P
中科院分区：
文献类型：
--
作者：
KARCH, F;GALLONI, M;SCHEDL, P
~ 300 kb的非常大的顺式调节区负责双胸复合体Ubx、abd-A和abd-B的三个同源异型基因的复杂表达模式。该区域可细分为9个副片段特异性调节亚基。最近的遗传和分子分析揭示了两个新的顺式调节元件Mcp和Fab-7的存在。Mcp位于iab-4和iab-5之间，iab-4和iab-5是指导第9和第10段中Abd-B的副片段特异性调节亚基。类似地，Fab-7位于iab-6和iab-7之间，即parasegment 11和12特异性调节单元。Mcp和Fab-7似乎起到分隔相邻顺式调节单元的结构域边界的作用。我们报告了两个新的Mcp突变体缺失（MCp（H27）和Mcp（B116）），使我们能够本地化的边界功能的一个类似的0.4 kb的DNA片段所必需的序列的分析。这些必需序列与染色质中一个约0.3kb的核酸酶超敏感区高度一致。我们还发现，有助于Fab-7边界的序列似乎分布在更大的DNA片段上，但与Mcp一样，它具有不寻常的染色质结构。
A very large cis-regulatory region of -300 kb is responsible for the complex patterns of expression of the three homeotic genes of the bithorax complex Ubx, abd-A and Abd-B. This region can be subdivided in nine parasegment-specific regulatory subunits. Recent genetic and molecular analysis has revealed the existence of two novel cis-regulatory elements Mcp and Fab-7. Mcp is located between iab-4 and iab-5, the parasegment-specific regulatory subunits which direct Abd-B in parasegments 9 and 10. Similarly, Fab-7 is located between iab-6 and iab-7, the parasegment 11 and 12-specific regulatory units. Mcp and Fab-7 appear to function as domain boundaries that separate adjacent cis-regulatory units. We report the analysis of two new Mcp mutant deletions (MCp(H27) and Mcp(B116)) that allow us to localize sequences essential for boundary function to a similar to 0.4 kb DNA segment. These essential sequences closely coincide to a similar to 0.3 kb nuclease hypersensitive region in chromatin. We also show that sequences contributing to the Fab-7 boundary appear to be spread over a larger stretch of DNA, but like Mcp have an unusual chromatin structure.