Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system.
Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system.
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DOI：
10.1038/s41598-021-87824-5
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发表时间：
2021-04-22
影响因子：
4.6
通讯作者：
Lai Z
中科院分区：
文献类型：
--
作者：
Zhang T;Niu Z;Wu F;Chen Z;Xu J;Jiang K;Lai Z
细菌培养和药物敏感性测试用于识别病原体感染。然而，从收集到鉴定细菌种类和耐药模式，这个过程需要几天的时间。数字PCR系统是一种快速发展的定量检测技术，广泛应用于分子诊断，包括拷贝数变异、单核苷酸变异分析、癌症生物标志物发现和病原体鉴定。本研究旨在利用液滴数字PCR系统识别血液样本中的细菌，并探讨其识别菌血症病原体的能力。然后，我们设计了大肠杆菌和金黄色葡萄球菌的SWG-9和COA基因的引物和探针，用ddPCR系统对血液样本进行鉴定。该系统在血样中表现出极高的检测精度，大肠杆菌检出率为13.1%~21.4%，金黄色葡萄球菌检出率为50%~88.3%。最后，对同时含有大肠杆菌和金黄色葡萄球菌的血液样本进行了检测，进一步评价了该方法的准确性和适用性，检出率在18.1%至97%之间。 ddPCR 系统作为快速检测病原体的定性和定量筛选方法非常有前景。
Bacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely applied to molecular diagnosis, including copy number variations, single nucleotide variant analysis, cancer biomarker discovery, and pathogen identification. This study aimed to use a droplet digital PCR system to identify bacteria in blood samples and explore its ability to identify pathogen in bacteremia. Then, we designed primers and probes of SWG-9 and COA gene for E. coli and S. aureus to identify in blood samples with the ddPCR system. The system had demonstrated extremely high detection accuracy in blood samples, and the detection rate of E. coli was 13.1–21.4%, and that of S. aureus was 50–88.3%. Finally, blood samples containing both E. coli and S. aureus were tested to evaluate further the accuracy and applicability of this method, indicating the detection rates range from 18.1% to 97%. The ddPCR system is highly promising as a qualitatively and quantitatively screening method for rapidly detecting pathogen.
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DOI：
10.1016/s0140-6736(18)32203-7
发表时间：
2018-11-10
期刊：
Lancet (London, England)
影响因子：
--
作者：
GBD 2017 Causes of Death Collaborators
通讯作者：
GBD 2017 Causes of Death Collaborators
影响因子：
4.3
作者：
Corbisier, Philippe;Bhat, Somanath;Emslie, Kerry R.
通讯作者：
Emslie, Kerry R.
影响因子：
2
作者：
Blais, Burton W.;Martinez-Perez, Amalia
通讯作者：
Martinez-Perez, Amalia
影响因子：
1
作者：
Shan, Kai;Li, Jianguo;Guo, Wei
通讯作者：
Guo, Wei
影响因子：
4.2
作者：
Herbert, MA;Beveridge, CJE;Saunders, NJ
通讯作者：
Saunders, NJ