REVERSIBLE GROWTH ARREST IN SIMIAN-VIRUS 40-TRANSFORMED HUMAN-FIBROBLASTS
REVERSIBLE GROWTH ARREST IN SIMIAN-VIRUS 40-TRANSFORMED HUMAN-FIBROBLASTS
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DOI：
10.1073/pnas.77.12.7306
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
JACOBSEN, SJ
中科院分区：
文献类型：
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作者：
HOFFMAN, RM;JACOBSEN, SJ
SV 40转化的人成纤维细胞的可逆生长停滞，由其直接代谢前体同型半胱氨酸取代生长培养基中的蛋氨酸产生。虽然这些被抑制的细胞在补充蛋氨酸的培养基中表现出大大降低的克隆效率，但当未融合的细胞用补充蛋氨酸的培养基处理时，它们恢复快速增殖而没有滞后。这种生长停滞伴随着细胞群中有丝分裂细胞百分比的减少。荧光激活的细胞分选技术表明，细胞停留在细胞周期的S期和G2期。这与G1期细胞聚集形成对比，G1期细胞聚集只发生在非常高密度的蛋氨酸补充培养基中，这与正常成纤维细胞在高密度培养中所见的G1块相似。讨论了这些转化培养中dna合成和细胞周期前有丝分裂阶段的特定事件与蛋氨酸依赖性之间的明显关系。
A reversible growth arrest of SV 40-transformed human fibroblasts as produced by replacement of methionine in the growth medium by its immediate metabolic precursor, homocysteine. Although these arrested cells exhibit a greatly reduced cloning efficiency when plated in methionine-supplemented medium, they resume rapid proliferation without a lag when subconfluent cells are refed with methionine-supplemented medium. This growth arrest is accompanied by a reduction in the percentage of mitotic cells in the cell population. Fluorescence-activated cell sorting techniques indicate that the cells are arrested in the S and G2 phases of the cell cycle. This is in contrast to a G1-phase accumulation of cells, which occurs only in methionine-supplemented medium at very high densities and which is similar to the G1 block seen in cultures of normal fibroblasts at high density. The apparent relationship between specific events in the DNA-synthetic and premitotic phase of the cell cycle and methionine dependence in these transformed cultures is discussed.