Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves
Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves
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DOI：
10.1093/oxfordjournals.pcp.a029397
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发表时间：
1998-05-01
影响因子：
4.9
通讯作者：
Ohashi, Y
中科院分区：
文献类型：
--
作者：
Niki, T;Mitsuhara, I;Ohashi, Y
水杨酸（SA）和茉莉酸（JA）分别是诱导表达酸性和碱性致病相关蛋白（PR）基因的病原菌和创口信号通路中必需的化合物。然而，关于外源供应SA和JA对PR基因表达的影响，在不同发育阶段的不同植物材料中得到了相互矛盾的结果，在这两种信号同时存在的情况下，没有明确的证据表明这些作用。我们分析了SA对伤口和JA诱导的碱性PR基因表达的影响，以及JA对SA诱导的酸性PR基因表达的影响。在MeJA的作用下，4种碱性PR基因的转录本积累均增强，而在SA的作用下则受到抑制，而3种酸性PR基因的表达均受到SA的诱导，而MeJA的作用则受到抑制。使用针对酸性PR-1和PR-2蛋白的抗体，这些作用在蛋白水平上得到证实。这些结果表明，JA是碱性PR基因的诱导剂，同时也是酸性PR基因的抑制剂，而SA则相反。
Salicylic acid (SA) and jasmonic acid (JA) are essential compounds in the pathogen-and wound-signaling pathways accompanying induced expression of acidic and basic pathogenesis-related (PR) protein genes, respectively. However, on the effect of exogenously supplied SA and JA in induction of PR gene expression, conflicting results have been obtained using various plant materials at different developmental stages, There is no clear evidence on these effects in the presence of both signals at the same time, We analyzed the effect of SA an wound- and JA-induced basic PR gene expression and that of JA on SA-induced acidic PR gene expression in mature tobacco leaves, Wound-induced accumulation of transcripts for all 4 basic PR genes tested was enhanced in the presence of MeJA, and inhibited in the presence of SA, On the other hand, expression of all 3 acidic PR genes tested was induced by SA and was inhibited by MeJA. Using antibodies raised against acidic PR-1 and PR-2 proteins, these effects were confirmed at the protein level. These results indicated that JA works as an inducer of basic PR genes, and also as an inhibitor for acidic PR genes, while SA does the opposite.