Ribosomal protein L18 is an essential factor that promote rice stripe virus accumulation in small brown planthopper
Ribosomal protein L18 is an essential factor that promote rice stripe virus accumulation in small brown planthopper
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核糖体蛋白L18是促进小褐飞虱体内水稻条纹病毒积累的重要因素
DOI：
10.1016/j.virusres.2018.01.011
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发表时间：
2018-03-02
期刊：
影响因子：
5
通讯作者：
Zhou, Yijun
中科院分区：
文献类型：
--
作者：
Li, Shuo;Li, Xing;Zhou, Yijun
由小褐飞虱 (SBPH) 传播的水稻条纹病毒 (RSV) 可引起严重的水稻病害。核衣壳（N）蛋白是RSV核糖核蛋白颗粒（RNP）的主要成分，在SBPH病毒持续繁殖传播中发挥着重要作用。为了进一步了解 RSV 传播的载体成分，利用基于 GAL4 的酵母双杂交系统来寻找与 N 蛋白相互作用的未知载体因子。十三种不同的蛋白质被确定为与 N 蛋白相互作用的因子。进一步研究了60S核糖体蛋白L18（RPL18）与N蛋白之间的相互作用。尽管 RPL18 的表达在 RSV 感染期间在昆虫中没有改变，但经验证，RPL18 可以直接与 RSV RNP 结合并与 RSV N 蛋白相互作用。 RPL18 的敲低显着降低了病毒 RNA 和蛋白质水平，尤其是病毒蛋白质表达，表明 RSV 翻译和复制需要 RPL18。我们的结果证明RPL18是SBPH中RSV积累所需的关键因子，这表明载体因子RPL18可能是控制水稻病毒传播的潜在靶标。
Rice stripe virus (RSV) transmitted by the vector, small brown planthopper (SBPH), can cause a severe rice disease. The nucleocapsid (N) protein is the major component of RSV ribonucleoprotein particles (RNPs), and it plays important roles in viral persistent-propagative transmission by SBPH. To gain further insights into the vector components enabling RSV transmission, a GAL4-based yeast two-hybrid system was utilized to find unknown vector factors that interact with the N protein. Thirteen different proteins were identified as factors that interact with the N protein. The interaction between 60S ribosomal protein L18 (RPL18) and the N protein was further studied. Although the expression of RPL18 was not altered in insects during RSV infection, RPL18 was validated to bind directly to RSV RNPs and interact with RSV N protein. Knockdown of RPL18 dramatically reduced viral RNA and protein levels, especially viral protein expression, indicating a requirement for RPL18 in RSV translation and replication. Our results provide evidence that RPL18 is a critical factor required for RSV accumulation in SBPH, which suggests that the vector factor RPL18 may be as a potential target to develop for controlling the transmission of rice virus.