Characterization of Bves expression during mouse development using newly generated immunoreagents
Characterization of Bves expression during mouse development using newly generated immunoreagents
复制标题
DOI：
10.1002/dvdy.20739
复制
发表时间：
2006-06-01
影响因子：
2.5
通讯作者：
Bader, David M.
中科院分区：
文献类型：
--
作者：
Smith, Travis K.;Bader, David M.
Bves（血管/心外膜物质）是一种跨膜蛋白，被认为在细胞-细胞相互作用/粘附中起作用。它作为一种在发育中的心脏中高度富集的基因产物被两组独立分离。在发展过程中对其表达存在分歧。最值得注意的是，Bves在非肌肉细胞中的表达存在争议。确定Bves的表达谱是表征发育和成人中蛋白质功能的关键一步。我们制备了新的抗小鼠Bves单克隆抗体，并利用这些免疫试剂来阐明Bves在发育过程中的表达。正如预期的那样，我们在整个心脏发育过程中检测到心肌细胞中的Bves。此外，包括冠状动脉系统在内的骨骼和平滑肌细胞也表达Bves。最后，特异性的，但不是全部的，三个胚层的上皮衍生物被这些单克隆抗体阳性染色。在培养的上皮细胞和肌肉细胞系中的蛋白表达证实了我们在体内的发现。综上所述，这些结果表明，在小鼠胚胎发生过程中，Bves在多种上皮细胞和肌肉细胞中表达，表明该蛋白在发育过程中具有广泛的功能，并表明这些新生成的试剂将对Bves的进一步研究具有宝贵的价值。
Bves (blood vessel/epicardial substance) is a transmembrane protein postulated to play a role in cell-cell interaction/adhesion. It was independently isolated by two groups as a gene product highly enriched in the developing heart. Disagreement exists about its expression during development. Most notably, the expression of Bves in non-muscle cells is disputed. Determining the expression profile of Bves is a critical initial step preceding the characterization of protein function in development and in the adult. We have generated new monoclonal antibodies against mouse Bves and used these immunoreagents to elucidate Bves expression in development. As expected, we detect Bves in myocytes of the developing heart throughout development. In addition, skeletal and smooth muscle cells including those of the coronary system express Bves. Finally, specific, but not all, epithelial derivatives of the three germ layers are stained positively with these monoclonal antibodies. Protein expression in cultured epithelial and muscle cell lines corroborate our in vivo findings. Taken together, these results demonstrate the expression of Bves in a wide range of epithelial and muscle cells during mouse embryogenesis and indicate a broad function for this protein in development, and show that these newly generated reagents will be invaluable in further investigation of Bves.