XERODERMA PIGMENTOSUM GROUP-E CELLS LACK A NUCLEAR FACTOR THAT BINDS TO DAMAGED DNA
XERODERMA PIGMENTOSUM GROUP-E CELLS LACK A NUCLEAR FACTOR THAT BINDS TO DAMAGED DNA
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DOI：
10.1126/science.3175673
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发表时间：
1988-10-28
期刊：
影响因子：
56.9
通讯作者：
CHANG, E
中科院分区：
文献类型：
--
作者：
CHU, G;CHANG, E
着色性干皮病的特征在于受损DNA的修复不足。来自不同患者的细胞融合已经定义了9个遗传互补组（A到I），这意味着人类的DNA修复涉及多种基因产物。在本报告中，使用凝胶电泳结合试验的扩展来鉴定至少一种核因子，该核因子（i）与紫外线辐射或抗肿瘤药物顺铂损伤的DNA结合，但（ii）在互补组E的细胞中明显缺失。因此，该因子似乎在结合和识别阶段参与多功能DNA修复途径。
The disease xeroderma pigmentosum is characterized by deficient repair of damaged DNA. Fusions of cells from different patients have defined nine genetic complementation groups (A through I), implying that DNA repair in humans involves multiple gene products. In this report, an extension of the gel electrophoresis binding assay was used to identify at least one nuclear factor that (i) bound to DNA damaged by ultraviolet radiation or the antitumor drug cisplatin, but (ii) was notably absent in cells from complementation group E. Therefore, the factor appears to participate in a versatile DNA repair pathway at the stage of binding and recognition.