Characterization of a highly variable eutherian rnicroRNA gene
Characterization of a highly variable eutherian rnicroRNA gene
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DOI：
10.1261/rna.2890305
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发表时间：
2005-08-01
期刊：
影响因子：
4.5
通讯作者：
Sharp, PA
中科院分区：
文献类型：
--
作者：
Houbaviy, HB;Dennis, L;Sharp, PA
小鼠microRNAs（miRNAs）miR-290-miR 295由一簇部分同源的pre-miRNA发夹编码，并且可能在胚胎干（ES）细胞和植入前胚胎中具有重要的功能。我们提出的证据表明，剪接，加帽和多腺苷酸化的初级转录跨越整个早期胚胎microRNA簇（EEmiRC）。部分Drosha加工产生额外的大核RNA中间体。含有TATA盒的保守启动子元件指导EEmiRC转录。序列分析表明，EEmiRC转录单位是显着可变的，只能在胎盘（真兽目）哺乳动物的生物信息学鉴定。与真兽特异性功能一致，EEmiRC在滋养层干细胞（TS）中表达。在分析进化和功能关系时，除了成熟的miRNA序列外，还应考虑整个miRNA基因座的组织。这一概念的应用表明，EEmiRC是一个最近获得的快速进化的基因真兽类的发展很重要。
Mouse microRNAs (miRNAs) miR-290-miR295 are encoded by a cluster of partially homologous pre-miRNA hairpins and are likely to be functionally important in embryonic stem (ES) cells and preimplantation embryos. We present evidence that a spliced, capped, and polyadenylated primary transcript spans this entire Early Embryonic microRNA Cluster (EEmiRC). Partial Drosha processing yields additional large nuclear RNA intermediates. A conserved promoter element containing a TATA-box directs EEmiRC transcription. Sequence analysis shows that the EEmiRC transcription unit is remarkably variable and can only be identified bioinformatically in placental (eutherian) mammals. Consistent with eutherian-specific function, EEmiRC is expressed in trophoblastic stem (TS) cells. When analyzing evolutionary and functional relationships, the organization of the entire miRNA loci should be considered in addition to the mature miRNA sequences. Application of this concept suggests that EEmiRC is a recently acquired rapidly evolving gene important for eutherian development.