Newcastle disease virus phosphoprotein gene analysis and transcriptional editing in avian cells
Newcastle disease virus phosphoprotein gene analysis and transcriptional editing in avian cells
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DOI：
10.1016/s0168-1702(00)00175-1
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发表时间：
2000-08-01
期刊：
影响因子：
5
通讯作者：
Seal, BS
中科院分区：
文献类型：
--
作者：
Locke, DP;Sellers, HS;Seal, BS
测定了来自23株不同年代和地理来源的纽卡斯尔病毒（NDV）的磷蛋白（P）基因的核苷酸序列。序列变异，同义取代占主导地位，发生在整个P基因。一个例外是含有转录编辑位点的保守中心区域。在NDV P基因mRNA中检测到四个G核苷酸的添加，可能会产生替代的开放阅读框。然而，在所有分离株中，只有一个读框内终止密码子存在单个G添加，这将允许潜在的V蛋白。第二个潜在的终止密码子不存在于P基因的一致序列中的所有分离物与一个以上的G核苷酸添加在编辑位点。这排除了这些分离株中可能存在的W蛋白。第二个潜在的替代性框内起始位点存在于所有分离株中，其可以编码NDV的预测X蛋白。副粘病毒亚科中的P基因编辑位点和预测的P基因使用的比较表明，NDV更接近于呼吸道病毒和麻疹病毒。通过对NDV分离株P基因序列的系统发育分析，发现NDV存在两个进化枝。一组包括1970年之前在美国分离的病毒，而第二组包括在世界范围内传播的强毒病毒。(C)出版社：Elsevier Science B.V.
Nucleotide sequence was determined for the phosphoprotein (P) gene from 23 Newcastle disease virus (NDV) isolates representing all defined pathotypes with different chronological and geographic origins. Sequence variation, with synonymous substitutions dominating, occurred throughout the P gene. An exception was a conserved central region containing the transcriptional editing site. Four G nucleotide additions were detected in NDV P gene mRNA potentially creating alternative open reading frames. However, only one in-frame stop codon exists with a single G addition among all isolates that would allow for a potential V protein. A second potential stop codon does not exist in the P gene consensus sequence among all isolates with more than one G nucleotide addition at the editing site. This precludes a possible W protein in these isolates. A second potential alternative in-frame start site exists among all isolates that could encode a predicted X protein for NDV. Comparison of the P gene editing sites among the Paramyxovirinae and predicted P gene usage demonstrates that NDV more closely resembles the respiroviruses and morbilliviruses. Phylogenetic analysis of P gene sequences among NDV isolates demonstrates there are two clades of these viruses. One group includes viruses isolated in the US prior to 1970, while a second cluster includes virulent viruses circulating worldwide. (C) 2000 Published by Elsevier Science B.V.