Geometric, osmotic, and membrane mechanical properties of density-separated human red cells.
Geometric, osmotic, and membrane mechanical properties of density-separated human red cells.
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DOI：
10.1182/blood.v59.6.1121.1121
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发表时间：
1982-06
期刊：
影响因子：
20.3
通讯作者：
O. Linderkamp;H. Meiselman
中科院分区：
文献类型：
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作者：
O. Linderkamp;H. Meiselman
尽管有证据表明整个红细胞（RBC）的变形能力在老化过程中降低，但与老化过程相关的相关特定性质变化的报告有限，且不完全一致。本研究的目的是评估可能导致这种变形能力降低的一些因素。使用微量移液器技术测量了来自5名健康成人供体的未分级、顶部（“年轻”）和底部（“年老”）RBC的几何、渗透和膜机械性质。在渗透压摩尔浓度为297、254、202和153 mosm/kg时测量RBC的表面积、体积和直径。两个膜的机械性能，表面剪切弹性模量（亩）和时间常数（TC）的粘弹性恢复，只在等渗介质中进行了研究。在每种渗透压下，底部细胞的体积和表面积比顶部细胞的体积和表面积低约25%。与顶部细胞相比，底部细胞随着渗透压摩尔浓度降低而表现出较小的体积增加;对于所有三个组，表面积随着渗透压摩尔浓度的变化而保持恒定。底部电池的表面积与体积比和最小圆柱直径基本上与顶部电池相同。然而，底部细胞的表面积指数（RBC的实际面积除以相同体积的球体的面积）和膨胀指数（最大体积除以实际体积）均显著低于顶部RBC。在所有3个RBC群体中，剪切弹性模量（mu）为约0.006达因/cm，表明使膜的一部分变形所需的力不随RBC老化而改变。底部RBC的粘弹性时间常数（tc）为0.148 +/- 0.020（SD）秒，顶部细胞为0.099 +/- 0.017秒。这种差异表明，在旧RBC中，膜变形后的形状恢复延迟。膜表面粘度（eta），计算为tc乘以mu的乘积，底部细胞为0.95 +/- 0.22 x 10（-3）达因-秒/cm，顶部RBC为0.54 +/- 0.15 x 10（-3）。这些数据表明，膜表面积的相对赤字和增加的膜粘度的老红细胞可能是重要的决定因素，为他们的变形能力下降，并最终从循环中删除。
Although there is evidence that the deformability of the entire red blood cell (RBC) decreases during aging, reports on changes in relevant specific properties associated with the aging process are limited and not in total agreement. The purpose of this study was to evaluate some of the factors that might contribute to this decreased deformability. Geometric, osmotic, and membrane mechanical properties of unfractionated, top ("young") and bottom ("old") RBC from 5 healthy adult donors were measured using micropipette techniques. Surface area, volume, and diameter of RBC were measured at osmolalities of 297, 254, 202, and 153 mosm/kg. Two membrane mechanical properties, surface shear modulus of elasticity (mu) and time constant (tc) of viscoelastic recovery, were studied only in isotonic media. At each of the osmolalities, volume and surface area of the bottom cells were about 25% lower than those of the top cells. Bottom cells showed smaller increases in volume with decreasing osmolality than top cells; the surface area remained constant with changing osmolality for all three groups. The surface area-to-volume ratio and the minimum cylindrical diameter of the bottom cells were essentially identical to the top cells. However, both the surface area index (actual are of RBC divided by area of a sphere of same volume) and the swelling index (maximal volume divided by actual volume) of the bottom cells were significantly lower than top RBC. The shear modules of elasticity (mu) was about 0.006 dyne/cm in all 3 RBC populations, indicating that the forces necessary to deform a portion of the membrane did not change with RBC aging. The viscoelastic time constant (tc) was 0.148 +/- 0.020 (SD) sec for the bottom RBC and 0.099 +/- 0.017 sec for the top cells. This difference indicates that shape recovery following membrane deformation is delayed in old RBC. The membrane surface viscosity (eta), calculated as the product of tc times mu was 0.95 +/- 0.22 x 10(-3) dyne-sec/cm for the bottom cells and 0.54 +/- 0.15 x 10(-3) for the top RBC. These data indicate that the relative deficit in membrane surface area and the increased membrane viscosity of old RBC may be important determinants for their decreased deformability and their eventual removal from the circulation.