Differential expression of FABP 3, 5, 7 in infantile and adult monkey cerebellum
Differential expression of FABP 3, 5, 7 in infantile and adult monkey cerebellum
复制标题
DOI：
10.1016/j.neures.2010.07.2028
复制
发表时间：
2010-10-01
影响因子：
2.9
通讯作者：
Yamashima, Tetsumori
中科院分区：
文献类型：
--
作者：
Boneva, Nadezhda B.;Mori, Yoshimi;Yamashima, Tetsumori
为了阐明脂肪酸结合蛋白（FABPs）在小脑发育和功能中的作用，我们通过比较幼龄、正常和发病后的成年猴子，研究了三种脑表达FABPs （fabp3、fabp5和fabp7）的分布。免疫印迹分析显示，在对照组和缺血后成人小脑中，fabp3和7表达强烈，而FABP5不表达。FABP7蛋白水平逐渐升高，而fabp3和fabp5蛋白水平则不升高，直至损伤后2周。免疫组化分析显示，小脑fabp3阳性细胞为浦肯野细胞和伯格曼胶质细胞。fabp5阳性细胞仅在缺血后小脑中发现，并被鉴定为活化的小胶质细胞。有趣的是，在婴儿小脑中，外颗粒层（EGL）的颗粒细胞祖细胞和内颗粒层（IGL）的少突胶质细胞祖细胞都表达FABP5。在成人小脑中，FABP7在浦肯野细胞和篮状中间神经元中表达，而在婴儿小脑中，也在伯格曼胶质细胞中发现。这些结果表明FABPs在小脑神经元和胶质细胞类型中的表达存在差异；fabp3和7主要在正常小脑中表达，fabpp5在缺血损伤后表达，而fabp3、5和7在小脑发育过程中表达。(C) 2010爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
To clarify the involvement of fatty acid binding proteins (FABPs) in cerebellar development and function, we explored the distribution of three brain-expressed FABPs, FABP 3,5 and 7, by comparing three animal groups - infantile, normal and postischemic adult monkeys. Immunoblotting analysis revealed intense expression of FABP 3 and 7, but not of FABP5, in the control and postischemic adult cerebellum. The protein levels of FABP7, but not of FABP 3 or 5, gradually increased until 2 weeks after the insult. Immunohistochemical analysis showed that cerebellar FABP3-positive cells were Purkinje cells and Bergmann glia. FABP5-positive cells were found only in the postischemic cerebellum, and were identified as activated microglia. Interestingly, in the infantile cerebellum, both the granule cell progenitors in the external granular layer (EGL) and the oligodendrocyte progenitors in the internal granular layer (IGL) expressed FABP5. In the adult cerebellum, FABP7 was expressed in Purkinje cells and basket interneurons, while in the infantile cerebellum it was also found in Bergmann glia. These results showed differential expression of FABPs in cerebellar neuronal and glial cell types; FABP 3 and 7 were predominantly expressed in normal cerebellum, FABP5 after ischemic injury, while FABP 3, 5 and 7 were expressed during cerebellar development. (C) 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.