Cell-mediated immune response following Mycoplasma pneumoniae infection in man. I. Lymphocyte stimulation.
Cell-mediated immune response following Mycoplasma pneumoniae infection in man. I. Lymphocyte stimulation.
复制标题
人类肺炎支原体感染后细胞介导的免疫反应。
DOI：
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发表时间：
1974
影响因子：
4.6
通讯作者：
G. Sterner
中科院分区：
文献类型：
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作者：
G. Biberfeld;P. Biberfeld;G. Sterner
来自17例近期肺炎支原体感染病例、13例既往感染病例和10例缺乏肺炎支原体抗体的健康受试者的淋巴细胞。肺炎支原体的各种抗原制剂的存在下培养。肺炎。通过[14C]胸苷的摄取和形态转化细胞的计数来测量淋巴细胞刺激。淋巴细胞对M.肺炎微生物和膜组分的表达在近期感染的患者中显著高于健康对照。然而，10个血清学阴性对照中有9个显示出一定程度的淋巴细胞对M.肺炎。M.在补体结合试验中具有高抗原滴度的肺炎克雷伯氏菌对感染病例或对照受试者的淋巴细胞没有明显的刺激作用。
淋巴细胞对超声处理的M.肺炎抗原可在感染后10年内得到证实。
在近期或既往M.肺炎支原体感染时，淋巴细胞刺激程度与抗体滴度无相关性。肺炎。
Lymphocytes from seventeen cases of recent Mycoplasma pneumoniae infection, thirteen cases of previous infection and ten healthy subjects lacking antibodies to M. pneumoniae were cultured in the presence of various antigen preparations of M. pneumoniae. Lymphocyte stimulation was measured by the uptake of [14C]thymidine and by counting of morphologically transformed cells. The lymphocyte response to a sonicated concentrate of M. pneumoniae organisms and to a membrane fraction was significantly higher in recently infected patients than in healthy controls. However, nine out of ten serologically negative controls showed some degree of lymphocyte reactivity to M. pneumoniae. A lipid fraction of M. pneumoniae, which had a high antigen titre in the complement fixation test, induced no appreciable stimulation of lymphocytes from infected cases or control subjects.
Lymphocyte responsiveness to sonicated M. pneumoniae antigen was demonstrable up to 10 years after infection.
Among the cases with recent or previous M. pneumoniae infection there was no correlation between the degree of lymphocyte stimulation and the titres of antibodies to M. pneumoniae.