EXPRESSION OF TAC ANTIGEN COMPONENT OF BOVINE INTERLEUKIN-2 RECEPTOR IN DIFFERENT LEUKOCYTE POPULATIONS INFECTED WITH THEILERIA-PARVA OR THEILERIA-ANNULATA
EXPRESSION OF TAC ANTIGEN COMPONENT OF BOVINE INTERLEUKIN-2 RECEPTOR IN DIFFERENT LEUKOCYTE POPULATIONS INFECTED WITH THEILERIA-PARVA OR THEILERIA-ANNULATA
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DOI：
10.1128/iai.58.12.3847-3855.1990
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发表时间：
1990-12-01
影响因子：
3.1
通讯作者：
MORRISON, WI
中科院分区：
文献类型：
--
作者：
DOBBELAERE, DAE;PROSPERO, TD;MORRISON, WI
牛白细胞介素-2受体的Tac抗原组分表达为Cro-β-半乳糖苷酶融合蛋白在大肠杆菌和用于提高抗体在兔子。这些抗体用于流式细胞荧光分析，以研究Tac抗原在各种小泰勒虫感染的细胞系中的表达，也在三个环形泰勒虫感染的细胞系中。在所有T.感染细小病毒的细胞系测试这些细胞是T细胞还是B细胞来源。表达Tac抗原的T细胞可以是CD 4-CD 8-、CD 4 + CD 8-、CD 4-CD 8+或CD 4 + CD 8+。Tac抗原的表达在实验室保存数年的培养物和最近用T. parva。北方（RNA）印迹分析表明，所有T.小孢子虫感染的细胞系。三个T还测试了非T细胞来源的annulata感染的细胞系。其中两个在其表面表达Tac抗原。在这些T. annulata感染的细胞系中，但只有微量的第三个细胞系中，其中含有非常少的Tac抗原表达细胞证明。在所有测试的细胞系中，无论是克隆的还是未克隆的，一部分细胞在其表面上不表达可检测水平的Tac抗原。许多其他白细胞表面标志物也是如此。此外，我们表明，白细胞介素-2受体的生物功能，因为除了重组白细胞介素-2培养刺激细胞增殖。重组白细胞介素-2治疗也导致稳态Tac抗原mRNA的量增加。泰勒虫感染细胞上白细胞介素2受体表达的相关性进行了讨论。
The Tac antigen component of the bovine interleukin-2 receptor was expressed as a Cro-.beta.-galactosidase fusion protein in Escherichia coli and used to raise antibodies in rabbits. These antibodies were used for flow cytofluorimetric analysis to investigate the expression of Tac antigen in a variety of Theileria parva-infected cell lines and also in three Theileria annulata-infected cells lines. Cells expressing Tac antigen on their surface were found in all T. parva-infected cell lines tested whether these were of T- or B-cell origin. T cells expressing Tac antigen could be CD4- CD8-, CD4+ CD8-, CD4- CD8+, or CD4+ CD8+. Tac antigen expression was observed both in cultures which had been maintained in the laboratory for several years and in transformed cells lines which had recently been established by infection of lymphocytes in vitro with T. parva. Northern (RNA) blot analysis demonstrated Tac antigen transcripts in RNA isolated from all T. parva-infected cell lines. Three T. annulata-infected cell lines which were not of T-cell origin were also tested. Two of them expressed Tac antigen on their surface. Abundant Tac antigen mRNA was detected in these T. annulata-infected cell lines, but only trace amounts were demonstrated in the third cell line, which contained very few Tac antigen-expressing cells. In all cell lines tested, whether cloned or uncloned, a proportion of the cells did not express detectable levels of Tac antigen on their surface. This was also the case for a number of other leukocyte surface markers. In addition, we showed that the interleukin-2 receptors were biologically functional, because addition of recombinant interleukin-2 to cultures stimulated cell proliferation. Recombinant interleukin-2 treatment also resulted in increased amounts of steady-state Tac antigen mRNA. The relevance of interleukin-2 receptor expression on Theileria-infected cells is discussed.