Strongyloides ratti infection induces transient nematode-specific Th2 response and reciprocal suppression of IFN-γ production in mice
Strongyloides ratti infection induces transient nematode-specific Th2 response and reciprocal suppression of IFN-γ production in mice
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DOI：
10.1111/j.1365-3024.2010.01199.x
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发表时间：
2010-05-01
影响因子：
2.2
通讯作者：
Breloer, M.
中科院分区：
文献类型：
--
作者：
Eschbach, M. -L.;Klemm, U.;Breloer, M.
P>世界上超过三分之一的人口感染了寄生蠕虫，类圆线虫亚种。大约有30-1亿感染者。在本研究中，我们利用鼠类圆线虫感染实验系统来研究这种致病线虫与其哺乳动物宿主的相互作用。我们提供了对鼠沙门氏菌感染的免疫反应的全面动力学描述，该反应通过抗原特异性 IgM 和 IgG1 的诱导、肥大细胞激活和 Th2 样细胞因子反应来反映。与源自初始小鼠的 T 细胞相比，源自受感染小鼠的 T 细胞对 CD3 接合的 IL-3、IL-4、IL-5、IL-13 和 IL-10 反应增强。 IFN-γ 对 CD3 结合的反应在原始小鼠的 T 细胞中可以很好地检测到，但在感染小鼠的 T 细胞中却受到抑制。鼠链球菌特异性 Th2 反应的诱导和促炎细胞因子的抑制都是短暂的，并且观察到与感染过程和使用的感染幼虫的数量严格相关。最后，将皮下注射幼虫诱导的人工感染与自然感染进行比较，我们观察到相似的抗原特异性 T 细胞反应，尽管自然感染导致蠕虫负担显着降低。
P>Over one-third of the world population is infected with parasitic helminths, Strongyloides ssp. accounting for approximately 30-100 million infected people. In this study, we employ the experimental system of murine Strongyloides ratti infection to investigate the interaction of this pathogenic nematode with its mammalian host. We provide a comprehensive kinetic description of the immune response to S. ratti infection that was reflected by induction of antigen-specific IgM and IgG1, mast cell activation and a Th2-like cytokine response. T cells derived from infected mice displayed an increased IL-3, IL-4, IL-5, IL-13 and IL-10 response to CD3-engagement in comparison with T cells derived from naive mice. The IFN-gamma response to CD3-engagement that was well detectable in T cells derived from naive mice, however, was suppressed in T cells derived from infected mice. Both, the induction of the S. ratti-specific Th2 response and the suppression of pro-inflammatory cytokines were transient and observed in strict correlation to the course of infection and the number of infective larvae used. Finally, comparing artificial infections induced by subcutaneous injection of larvae to natural infections, we observed similar antigen-specific T cell responses although the natural infection led to a significantly lower worm burden.