The Diversity of Torque Teno Viruses: In Vitro Replication Leads to the Formation of Additional Replication-Competent Subviral Molecules
The Diversity of Torque Teno Viruses: In Vitro Replication Leads to the Formation of Additional Replication-Competent Subviral Molecules
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DOI：
10.1128/jvi.02472-10
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发表时间：
2011-07-01
影响因子：
5.4
通讯作者：
Fei, Jian-Wei
中科院分区：
文献类型：
--
作者：
de Villiers, Ethel-Michele;Borkosky, Silvia S.;Fei, Jian-Wei
无绒病毒科包括具有不同基因组结构和组织的转矩病毒（ttv）。报道了26种TTV类型的大量TTV基因组（TTV Heidelberg [TTV- hd]）的分离。来自同一类型的几个分离株在开放阅读框1 （ORF1）中显示序列变异，导致开放阅读框明显改变。我们在293TT细胞中证明了TTV-HD的12个全长基因组的体外复制。病毒的繁殖是通过使用最初感染细胞的上清和冷冻的整个细胞进行几轮感染来实现的。通过PCR扩增和转录分析检测病毒复制。亚基因组分子（mu TTV）在繁殖早期产生，大小在401 ~ 913个碱基之间。这些mu TTV在没有全长基因组的情况下在体外培养中繁殖。
The family Anelloviridae comprises torque teno viruses (TTVs) diverse in genome structure and organization. The isolation of a large number of TTV genomes (TTV Heidelberg [TTV-HD]) of 26 TTV types is reported. Several isolates from the same type indicate sequence variation within open reading frame 1 (ORF1), resulting in considerably modified open reading frames. We demonstrate in vitro replication of 12 full-length genomes of TTV-HD in 293TT cells. Propagation of virus was achieved by several rounds of infections using supernatant and frozen whole cells of initially infected cells. Replication of virus was measured by PCR amplification and transcription analyses. Subgenomic molecules (mu TTV), arising early during propagation and ranging in size from 401 to 913 bases, were cloned and characterized. Propagation of these mu TTV in in vitro cultures was demonstrated in the absence of full-length genomes.