Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis
Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis
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DOI：
10.1007/s11418-017-1168-4
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发表时间：
2018-01
影响因子：
3.3
通讯作者：
Y. Yamamura;Yukari Taguchi;Kei Ichitani;I. Umebara;Ayako Ohshita;F. Kurosaki;Jung-Bum Lee
中科院分区：
文献类型：
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作者：
Y. Yamamura;Yukari Taguchi;Kei Ichitani;I. Umebara;Ayako Ohshita;F. Kurosaki;Jung-Bum Lee
赤霉素（GAs）是高等植物中普遍存在的二萜类化合物，而某些高等植物产生独特的种特异性二萜类化合物。在赤霉素生物合成中，对映贝壳杉烯合酶（KS）和对映贝壳杉烯氧化酶（KO）是催化环化和氧化反应的关键酶。本文研究了参与赤霉素生物合成的一个KS（SdKS）和两个科斯（SdKO 1和SdKO 2）基因产物的功能。利用大肠杆菌的体内异源表达系统，我们发现SdKS催化-CPP到对映贝壳杉烯的环化反应，并且SdKOs在N-末端区域被修饰以适应E.大肠杆菌表达系统实时荧光定量PCR结果表明，SdKS、SdKO 1和SdKO 2基因主要在根和侧根系统中表达，而侧根系统是根的伸长组织。基于这些结果，我们认为这三个基因可能是负责GAs在甜链霉菌中的代谢。
Gibberellins (GAs) are ubiquitous diterpenoids in higher plants, whereas some higher plants produce unique species-specific diterpenoids. In GA biosynthesis,ent-kaurene synthase (KS) andent-kaurene oxidase (KO) are key players which catalyze early step(s) of the cyclization and oxidation reactions. We have studied the functional characterization of gene products of a KS (SdKS) and two KOs (SdKO1andSdKO2) involved in GA biosynthesis inScoparia dulcis. Using an in vivo heterologous expression system ofEscherichia coli, we found that SdKS catalyzed a cyclization reaction froment-CPP toent-kaurene and that the SdKOs oxidizedent-kaurene toent-kaurenoic acid after modification of the N-terminal region for adaptation to theE. coliexpression system. The real-time PCR results showed that theSdKS,SdKO1andSdKO2genes were mainly expressed in the root and lateral root systems, which are elongating tissues. Based on these results, we suggest that these three genes may be responsible for the metabolism of GAs inS. dulcis.