Effect of intraventricular injection of dopamine, noreprinephrine, acetylcholine, and 5-hydroxytryptamine on immunoreactive somatostatin release into rat hypophyseal portal blood.
Effect of intraventricular injection of dopamine, noreprinephrine, acetylcholine, and 5-hydroxytryptamine on immunoreactive somatostatin release into rat hypophyseal portal blood.
复制标题
脑室内注射多巴胺、去甲肾上腺素、乙酰胆碱和 5-羟色胺对免疫反应性生长抑素释放到大鼠垂体门静脉血中的影响。
作者：
K. Chihara;A. Arimura;A. V. Schally
在乌拉坦麻醉的雄性大鼠中，研究了几种假定的神经递质在调节生长抑素从正中隆起释放到垂体门静脉血中的作用。使用专门设计的微量组分收集器，以15分钟的间隔将垂体门静脉血收集到5个含有2 N乙酸的单独试管中。采用丙酮提取、有机溶剂洗涤、放免法测定酸性血液混合液中的免疫反应性生长抑素（IRS）。在采集第一份血样后，立即向第三脑室注射多巴胺、去甲肾上腺素、5-羟色胺或乙酰胆碱，剂量为10-8 mol/5 μl新鲜制备的5%葡萄糖溶液或单独的5 nl葡萄糖溶液。对照组10只，注射5%葡萄糖前IRS的分泌速率和浓度分别为3.90 ± 0.66 pg/min和628 ± 126 pg/ml，注射后15 min分别为2.12 ± 0.28 pg/min和286 ± 22 pg/ml，注射后15 min分别为1.93 ± 0.17 pg/min和261 ± 18 pg/ml。
The effect of several putative neurotransmitters in the regulation of somatostatin release from the median eminence into hypophyseal portal blood was investigated in male rats under urethane anesthesia. Using a specially designed microfraction collector, hypophyseal portal blood was collected at 15-min intervals into five separate tubes containing 2 N acetic acid. Immunoreactive somatostatin (IRS) in the acid-blood mixtures was extracted with acetone, washed with organic solvents, and determined by RIA. Dopamine, norepinephrine, 5-hydroxytryptamine, or acetylcholine in a dose of 10-8 mol/5 μl freshly prepared 5% glucose solution or 5 nl glucose solution alone was injected into the third ventricle immediately after collection of the first blood sample. In 10 control animals, the mean (±SE) secretion rate and concentration of IRS were 3.90=0.66 pg/min and 628 ± 126 pg/ml before injection of 5% glucose, 2.12 ± 0.28 pg/min and 286 ± 22 pg/ml 15 min after the injection, 1.93 ± 0.17 pg/min and 261 ± 18 pg/ml at...