Immobilization stress elevates gene expression for catecholamine biosynthetic enzymes and some neuropeptides in rat sympathetic ganglia: Effects of adrenocorticotropin and glucocorticoids
Immobilization stress elevates gene expression for catecholamine biosynthetic enzymes and some neuropeptides in rat sympathetic ganglia: Effects of adrenocorticotropin and glucocorticoids
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DOI：
10.1210/en.137.12.5597
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发表时间：
1996-12-01
期刊：
影响因子：
4.8
通讯作者：
Sabban, EL
中科院分区：
文献类型：
--
作者：
Nankova, B;Kvetnansky, R;Sabban, EL
交感神经节是应激引起的循环去甲肾上腺素、脑啡肽和神经肽 Y 升高的主要贡献者。在这里，我们检查了固定应激以及 ACTH 和糖皮质激素治疗对酪氨酸羟化酶 (TH)、多巴胺 β-羟化酶 (DBH)、 大鼠颈上神经节 (SCG) 和星状神经节中的前神经肽 Y (pre-NPY) 和脑啡肽原。我们的结果显示，单次固定后 TH 和 NPY mRNA 的相对丰度增加了数倍。重复的应激会提高所有研究基因的表达，并增加两个神经节的 TH 免疫反应性。压力的影响在 SCG 中更为明显。延长皮质醇给药未能改变对照动物的 TH、DBH 和 NPY mRNA 水平，但减弱了对应激的反应。相反，给予 ACTH 后，SCG 中的 TH 和 DBH mRNA 水平升高，但肾上腺髓质中未升高，与固定后达到的水平相似。结果表明，交感神经元响应固定应激的基因表达调节与肾上腺髓质的调节不同。该研究表明激素参与应激诱导的交感神经节 TH、DBH、NPY 和脑啡肽原基因表达的变化。
Sympathetic ganglia are the major contributors to the stress-elicited rise in circulating norepinephrine, enkephalins, and neuropeptide Y. Here we examined the effect of immobilization stress and treatment with ACTH and glucocorticoids on messenger RNA (mRNA) levels for tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), preproneuropeptide Y (pre-NPY), and proenkephalin in rat superior cervical ganglia(SCG) and in stellate ganglia. Our results show a severalfold increase in the relative abundance of TH and NPY mRNAs in response to a single immobilization. Repeated stress elevated expression of all the genes studied and increased TH immunoreactivity in both ganglia. The effect of stress was more pronounced in SCG. Prolonged cortisol administration failed to alter the mRNA levels of TH, DBH, and NPY in control animals but attenuated the response to stress. In contrast, TH and DBH mRNA levels in the SCG, but not in adrenal medulla, were elevated by ACTH administration, similar to the levels attained after immobilization. The results revealed that the regulation of gene expression in response to immobilization stress in sympathetic neurons differs from the regulation in adrenal medulla. The study implicates hormonal involvement in the stress-induced changes in TH, DBH, NPY, and proenkephalin gene expression in sympathetic ganglia.