Changes in gait after bilateral meniscectomy in sheep: effect of two hyaluronan preparations
Changes in gait after bilateral meniscectomy in sheep: effect of two hyaluronan preparations
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DOI：
10.1007/s00776-008-1279-6
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发表时间：
2008-11-01
影响因子：
1.7
通讯作者：
Ghosh, Peter
中科院分区：
文献类型：
--
作者：
Cake, Martin;Read, Richard;Ghosh, Peter
本研究检测了双侧肱骨切除术对绵羊地面反作用力（GRF）的影响，以及两种透明质酸（HA）制剂的治疗效果。18只绵羊接受双侧外侧肱骨切除术，术后16 - 20周关节内给予Hyalgan（Fidia Farmaceutical）、HYADD 4-G（一种新型酰胺衍生物; Fidia Farmaceutical）或生理盐水安慰剂（每组n = 6）治疗。在基线和术后6、12、16、22和26周评估GRF。半月板切除术可显著降低GRF，并消除正常的双峰向量。两种HA制剂均部分改善了GRF缺陷：Hyalgan在治疗后6周（第22周）增加了峰值垂直力，而HYADD 4-G在治疗后增加了垂直冲量。两种HA治疗，但不是生理盐水安慰剂，在治疗后6周恢复了双峰复合力矢量。HA制剂均未显著调节骨关节炎（OA）的严重程度或滑液参数。本研究表明，绵羊双侧骨关节炎切除术的GRF反应与人类骨关节炎切除术和OA患者的可用数据相似。然而，这一时间进程表明，步态缺陷是暂时无关的观察到的软骨或滑液的变化。双侧绵羊坐骨神经切除术模型显示出GRF的适度但可量化的变化，其模拟人类OA，并且适合于通过已知的OA疗法如HA进行修饰。
This study examined the effect of bilateral meniscectomy on ground reaction forces (GRFs) in sheep, and the therapeutic effect of two hyaluronan (HA) preparations.Eighteen sheep were subjected to bilateral lateral meniscectomy and were treated from 16 to 20 weeks postoperatively with intraarticular Hyalgan (Fidia Farmaceutici), HYADD4-G (a novel amide derivative; Fidia Farmaceutici), or saline placebo (n = 6 per group). GRFs were assessed at baseline and 6, 12, 16, 22, and 26 weeks postoperatively. Rheological parameters and HA content of synovial fluid samples were assessed using micro-Fourier rheometry.Meniscectomy significantly reduced GRF and abolished the normal two-peak vector. GRF deficits were partially ameliorated by both HA preparations: Hyalgan increased peak vertical forces at 6 weeks post-treatment (week 22), while HYADD4-G increased vertical impulse post-treatment. Both HA treatments, but not saline placebo, restored a twopeak composite force vector at 6 weeks post-treatment. Neither HA preparation significantly modulated osteoarthritis (OA) severity, or synovial fluid parameters.This study showed that GRF responses to bilateral meniscectomy in sheep mimic available data for human meniscectomy and OA patients. However, this time course suggests that gait deficits are temporally unrelated to observed cartilage or synovial fluid changes. The bilateral ovine meniscectomy model demonstrates modest but quantifiable changes in GRF that mimic human OA and are amenable to modification by known OA therapies such as HA.