Accelerated detection and identification of mycobacteria with MGIT 960 and COBAS AMPLICOR systems
Accelerated detection and identification of mycobacteria with MGIT 960 and COBAS AMPLICOR systems
复制标题
DOI：
10.1128/jcm.38.3.960-964.2000
复制
发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Erkinjuntti-Pekkanen, R
中科院分区：
文献类型：
--
作者：
Katila, ML;Katila, P;Erkinjuntti-Pekkanen, R
在临床常规中，将分枝杆菌自动培养系统MGIT 960系统（MGIT系统）与Lowenstein詹森（L-J）培养基的两种变体进行了比较。从2，015份标本中共回收152株分离株：MGIT系统139株（91%），L-J培养基127株（84%）（P = 0.05）。其中包括68株结核分枝杆菌分离株，其中88%在MGIT系统中生长，93%在L-J培养基中生长（P = 0.389），以及84株分枝杆菌以外的分枝杆菌分离株。结核病（MOTT），其中94%在MGIT系统中生长，76%在L-J培养基中生长（P = 0.003）。更多M. MGIT系统（n = 65）比L-J培养基（n = 50）检出禽流感复合群（P = 0.001）。MGIT系统中的生长在2周内检测到78%的分离株，而在两种L-J培养基中分别检测到17%和25%的分离株生长。MGIT系统检出结核分枝杆菌的平均时间为12天，L-J培养基为20天。平均检出时间分别为8天和22 ~ 25天。所有培养基的污染率相似（8.7 - 8.9%）。评价了商品化扩增系统（COBAS AMPLYCOR）快速鉴定M. tuberculosis，M. avium和M.直接从MGIT系统肉汤中的393个样品中获得胞内酶。通过培养或临床数据评价，96%的样品获得了正确的PCR结果，2%的样品检测到抑制。在89个阳性结果中，M.结核病，91%被认为是真阳性，8%被认为是不确定的，2%被认为是假阳性。对于M阳性结果。avium和M.细胞内的MOTT分别为97%和79%，被认为是真阳性。COBAS-AMPENDIX-COR适用于MGIT系统肉汤中这3种常见病原菌的快速鉴定。
An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Lowenstein Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These included 68 isolates of Mycobacterium tuberculosis, of which 88% grew in the MGIT system and 93% grew in L-J media (P = 0.389), and 84 isolates of mycobacteria other than M. tuberculosis (MOTT), of which 94% grew in the MGIT system and 76% grew in L-J media (P = 0.003). More M. avium complex isolates were detected in the MGIT system (n = 65) than in L-J media (n = 50) (P = 0.001). Growth in the MGIT system was detected in 2 weeks for 78% of the isolates, whereas growth was detected in the two L-J media for 17 and 25% of the isolates, respectively. The mean times to detection of M, tuberculosis were 12 days in the MGIT system and 20 days in L-J media, and for M. avium complex the mean times to detection were 8 and 22 to 25 days, respectively. The contamination rates were similar (8.7 to 8.9%) in all media. A commercial amplification system (COBAS AMPLICOR) was evaluated for its ability to rapidly identify M. tuberculosis, M. avium, and M. intracellulare directly from 393 samples in MGIT system broth. A correct PCR result, as evaluated by culture or clinical data, was obtained for 96% of the samples, with inhibition being detected for 2% of the samples. Of the 89 results positive for M. tuberculosis, 91% were regarded as true positive, 8% were regarded as inconclusive, and 2% were considered false positive. For results positive for M. avium and M. intracellulare, 97 and 79%, respectively, were regarded as true positive, Increased rapidity and enhanced isolation of MOTT were obtained with the MGIT system. COBAS AMPLICOR was suitable for rapid identification of these three common pathogens from MGIT system broth.