Production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) using aeromonas hydrophila 4AK4 grown in mixed carbon source
Production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) using aeromonas hydrophila 4AK4 grown in mixed carbon source
复制标题
使用混合碳源中生长的嗜水气单胞菌 4AK4 生产聚（3-羟基丁酸酯-co-3-羟基己酸酯）
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Guoqiang Chen
中科院分区：
文献类型：
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作者：
Jin Zhang;Guang Zhang;Jin‐chun Chen;Xiu;Guoqiang Chen
以大豆油和月桂酸为混合基质，培养嗜水气单胞菌4AK 4，制备3-羟基丁酸酯（3 HB）和3-羟基己酸酯（3 HHx）的聚羟基脂肪酸酯共聚物。在摇瓶培养条件下，干细胞中聚（3-羟基丁酸-3-羟基己酸）（PHBHHx）的含量最高可达49.13%。以100 g/L大豆油为单一碳源，发酵48 h，PHBHHx产量为6.26 g/L，以80 g/L大豆油和20 g/L月桂酸为混合碳源，发酵48 h，PHBHHx产量为12.40 g/L，聚羟基脂肪酸酯（PHA）产量为0.25 g/（L.h）。结果表明，混合碳源适合于A.作为混合碳源的Al 2 O3 4AK 4还克服了在PHBHHx生产中使用月桂酸作为唯一碳源时出现的起泡问题。
Aeromonas hydrophila 4AK4 was grown on mixed substrates of soybean oil and lauric acid for the production of polyhydroxyalkanoate copolymer consisting of 3 hydroxybutyrate (3HB) and 3 hydroxyhexanoate (3HHx). A maximal poly(3 hydroxybutyrate co 3 hydroxyhexanoate) (PHBHHx) content of 49.13% in dry cells was obtained in a shake flask culture. PHBHHx of 6.26 g/L was produced in a fermentation experiment over 48 h on a sole carbon source containing 100 g/L soybean oil, while 12.40 g/L PHBHHx was produced on a mixed carbon source containing 80 g/L soybean and 20 g/L lauric acid over the same period of time, resulting in a polyhydroxyalkanoate (PHA) productivity of 0.25 g/(L·h). The results show that mixed carbon sources are suitable for industrialized production of PHBHHx from A. hydrophila 4AK4, as the mixed carbon sources also overcome the foaming problem that occurs when lauric acid is employed as a sole carbon source in PHBHHx production.