Cell-cell interaction in vitro: studied by density separation of colony-forming, stimulating, and inhibiting cells from human bone marrow.
Cell-cell interaction in vitro: studied by density separation of colony-forming, stimulating, and inhibiting cells from human bone marrow.
复制标题
体外细胞间相互作用：通过人骨髓中集落形成、刺激和抑制细胞的密度分离进行研究。
DOI：
10.1182/blood.v40.3.394.394
复制
发表时间：
1972
期刊：
影响因子：
20.3
通讯作者：
P. Galbraith
中科院分区：
文献类型：
--
作者：
J. Haskill;R. D. McKnight;P. Galbraith
通过根据牛血清白蛋白线性梯度中的浮力密度将正常人骨髓细胞分离成级分，并通过在不添加外源集落刺激因子的甲基纤维素培养基中测定骨髓级分和骨髓级分组合的集落形成能力来研究体外细胞-细胞相互作用。在该培养系统中，未分级的骨髓细胞很少形成集落，但集落从低密度级分中生长，表明抑制剂已通过细胞分级去除。当来自低密度部分的细胞与来自中密度和高密度部分的细胞混合时，分别观察到集落大小的刺激和抑制。这些观察结果表明，细胞间相互作用可能在体外调节粒细胞生成中发挥作用，并提出了一种研究疾病中粒细胞生成的方法。
Cell-cell interaction in vitro was studied by separating normal human bone marrow cells into fractions on the basis of their buoyant density in a linear gradient of bovine serum albumin and by assaying the colony forming ability of marrow fractions and combinations of marrow fractions in methylcellulose medium without an added extrinsic source of colony-stimulating factor. In this culture system unfractionated marrow cells seldom formed colonies, but colonies grew from the low-density fractions, suggesting that an inhibitor had been removed by cell fractionation. When cells from the low-density fraction were mixed with the cells from the middensity and highdensity fractions, stimulation and inhibition of colony size respectively were observed. These observations suggest that cell-cell interaction may play a role in regulation of granulopoiesis in vitro and suggests an approach to the investigation of granulopoiesis in disease.