Casein and transglutaminase-mediated modification of wool surface
Casein and transglutaminase-mediated modification of wool surface
复制标题
酪蛋白和转谷氨酰胺酶介导的羊毛表面修饰
DOI：
10.1002/elsc.201000110
复制
发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Fu, Guangtong
中科院分区：
文献类型：
--
作者：
Cui, Li;Fan, Xuerong;Fu, Guangtong
在这项研究中，酪蛋白，牛奶中发现的蛋白质，被用作表面涂层材料，以改善羊毛织物的性能由微生物蛋白质交联酶：转氨酶（TGase）介导。对于本研究中使用的羊毛织物，4%的酪蛋白（owf）处理1小时结合20 U/g织物的微生物TGase减少了KMnO 4预处理的羊毛织物的面积收缩率从11.3 +/- 70.26到4.57 +/-0.15%。拉伸强度从275 +/- 5.0增加到315 +/- 2.1 N。在温水、Na_2CO_3和SDS溶液中，TGase交联的酪蛋白在羊毛上的稳定性优于灭活酶。扫描电子显微照片表明，酪蛋白和TGase处理通过涂覆或填充羊毛的凸起鳞片使羊毛纤维表面平滑。
In this study, casein, a protein found in milk, was used as a surface-coating material for improving wool fabric properties mediated by a microbial protein cross-linking enzyme: transglutaminase (TGase). For the wool fabric used in this study, 4% casein (owf) treatment for 1 h combined with 20 U/g fabric of microbial TGase reduced the area shrinkage of the KMnO4-pretreated wool fabric from 11.3 +/- 70.26 to 4.57 +/- 0.15%. The tensile strength increased from 275 +/- 5.0 to 315 +/- 2.1 N. The casein cross-linked by TGase on wool exhibited better stability than that with the inactivated enzyme in warm water, Na2CO3 and SDS solutions. Scanning electron micrographs demonstrated that the casein and TGase treatment smoothened the wool fiber surface by coating or filling the raised scales of the wool.