IMMUNO-CYTOCHEMICAL DEMONSTRATION OF MONOAMINE OXIDASE-B IN BRAIN ASTROCYTES AND SEROTONERGIC NEURONS
IMMUNO-CYTOCHEMICAL DEMONSTRATION OF MONOAMINE OXIDASE-B IN BRAIN ASTROCYTES AND SEROTONERGIC NEURONS
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DOI：
10.1073/pnas.79.20.6385
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
BREAKEFIELD, XO
中科院分区：
文献类型：
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作者：
LEVITT, P;PINTAR, JE;BREAKEFIELD, XO
用单胺氧化酶B(MAO-B)抗血清免疫细胞化学方法研究了这种代谢酶在成年大鼠脑内的分布。MAO-B定位于两大类中枢神经系统细胞，即星形胶质细胞和含5-羟色胺神经元。用胶质纤维酸性蛋白和MAO-B的抗血清进行的双重免疫荧光实验表明，大脑中的原浆和纤维星形胶质细胞都含有MAO-B，而少突胶质细胞不含有这种酶。缺乏血脑屏障的区域，如特殊的脑室周围器官，也含有MAO-B阳性细胞。用5-羟色胺和MAO-B的抗血清进行的双重免疫荧光实验使含有这两种分子的神经元得到了肯定的识别。大脑中含有儿茶酚胺的神经元不含有可检测到的MAO-B。MAO-B在成年中枢神经系统中的特殊分布表明，MAO-B在单胺代谢中的作用可能比先前认为的更具体。
An antiserum to monoamine oxidase B (MAO-B) was used to define the distribution of this metabolic enzyme in the adult rat brain immunocytochemically. MAO-B is specifically located in 2 major CNS cell classes, astrocytes and serotonin-containing neurons. Double-immunofluorescence experiments using antisera to glial fibrillary acidic protein and MAO-B showed that both protoplasmic and fibrillary astrocytes throughout the brain contain MAO-B, whereas oligodendrocytes do not contain the enzyme. Areas lacking a blood-brain barrier, such as the specialized circumventricular organs, also contain MAO-B-positive cells. A double-immunofluorescence experiment using antisera to serotonin and MAO-B enabled the positive identification of neurons containing both molecules. The catecholamine-containing neurons of the brain did not contain detectable amounts of MAO-B. The specific distribution of MAO-B in the adult CNS indicates that the role of MAO-B in monoamine metabolism may be more specifically defined than previously believed.