Clathrin-dependent and -independent internalization of plasma membrane sphingolipids initiates two Golgi targeting pathways.
Clathrin-dependent and -independent internalization of plasma membrane sphingolipids initiates two Golgi targeting pathways.
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DOI：
10.1083/jcb.200102084
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发表时间：
2001-08-06
期刊：
影响因子：
--
通讯作者：
Pagano RE
中科院分区：
文献类型：
--
作者：
Puri V;Watanabe R;Singh RD;Dominguez M;Brown JC;Wheatley CL;Marks DL;Pagano RE
Sphingolipids (SLs) are plasma membrane constituents in eukaryotic cells which play important roles in a wide variety of cellular functions. However, little is known about the mechanisms of their internalization from the plasma membrane or subsequent intracellular targeting. We have begun to study these issues in human skin fibroblasts using fluorescent SL analogues. Using selective endocytic inhibitors and dominant negative constructs of dynamin and epidermal growth factor receptor pathway substrate clone 15, we found that analogues of lactosylceramide and globoside were internalized almost exclusively by a clathrin-independent (“caveolar-like”) mechanism, whereas an analogue of sphingomyelin was taken up approximately equally by clathrin-dependent and -independent pathways. We also showed that the Golgi targeting of SL analogues internalized via the caveolar-like pathway was selectively perturbed by elevated intracellular cholesterol, demonstrating the existence of two discrete Golgi targeting pathways. Studies using SL-binding toxins internalized via clathrin-dependent or -independent mechanisms confirmed that endogenous SLs follow the same two pathways. These findings (a) provide a direct demonstration of differential SLs sorting into early endosomes in living cells, (b) provide a “vital marker” for endosomes derived from caveolar-like endocytosis, and (c) identify two independent pathways for lipid transport from the plasma membrane to the Golgi apparatus in human skin fibroblasts.
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DOI：
10.1083/jcb.121.1.61
发表时间：
1993-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hansen SH;Sandvig K;van Deurs B
通讯作者：
van Deurs B
DOI：
10.1083/jcb.108.6.2169
发表时间：
1989-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Koval M;Pagano RE
通讯作者：
Pagano RE
影响因子：
7.8
作者：
Koval, M;Pagano, R E
通讯作者：
Pagano, R E
DOI：
10.1083/jcb.114.2.231
发表时间：
1991-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kok JW;Babia T;Hoekstra D
通讯作者：
Hoekstra D
影响因子：
3.7
作者：
Aoki, T;Nomura, R;Fujimoto, T
通讯作者：
Fujimoto, T