Cloning of Epidermal Growth Factor (EGF) and EGF Receptor from the Zebrafish Ovary: Evidence for EGF as a Potential Paracrine Factor from the Oocyte to Regulate Activin/Follistatin System in the Follicle Cells1
Cloning of Epidermal Growth Factor (EGF) and EGF Receptor from the Zebrafish Ovary: Evidence for EGF as a Potential Paracrine Factor from the Oocyte to Regulate Activin/Follistatin System in the Follicle Cells1
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DOI：
10.1095/biolreprod.104.028399
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发表时间：
2004-09
期刊：
影响因子：
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通讯作者：
Yajun Wang;W. Ge
中科院分区：
文献类型：
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作者：
Yajun Wang;W. Ge
摘要本研究从斑马鱼卵巢中克隆了表皮生长因子（EGF）、表皮生长因子受体（EGFR）和三种截短型EGFR（EGFR 15、12和8）的全长cDNA。斑马鱼表皮生长因子主要表达在卵巢和睾丸，而表皮生长因子受体及其截短的形式在所有组织检查，除了肝脏高度表达。在卵巢中，EGF的表达似乎在早期阶段的卵泡中更丰富，而EGFR在晚期阶段的表达水平更高。有趣的是，尽管EGF在卵泡细胞和卵母细胞中均表达，但其在卵母细胞中的表达水平显著较高。EGFR主要在卵泡细胞中表达，在卵母细胞中表达较少。EGF和EGFR在卵泡内表达的独特空间模式表明EGF可能作为来自卵母细胞的信使向卵泡细胞发出信号。EGF可显著刺激激活素βA和βB的表达，而抑制卵泡抑素的基础表达和hCG诱导的卵泡抑素表达。这些结果，以及EGF主要在卵母细胞中表达而EGFR在卵泡细胞中表达的证据，强烈表明EGF可能是来自卵母细胞的潜在旁分泌/旁分泌因子，以调节卵泡细胞的功能。
Abstract In the present study, we cloned full-length cDNAs for epidermal growth factor (EGF), EGF receptor (EGFR), and three truncated forms of EGFR (EGFR15, 12, and 8) from the zebrafish ovary. Zebrafish EGF was predominantly expressed in the ovary and testis, while EGFR and its truncated forms were highly expressed in all tissues examined except the liver. In the ovary, the expression of EGF seemed to be more abundant in the follicles of early stages, while EGFR had much higher expression levels at later stages. Interestingly, although EGF was expressed in both the follicle cells and oocytes, its expression level was significantly higher in the oocytes. However, the expression of EGFR was mainly restricted to the follicle cells with little expression in the oocytes. The unique spatial patterns of EGF and EGFR expression within the follicle suggest that EGF may serve as a messenger from the oocyte to signal the follicle cells. EGF strongly stimulated the expression of both activin βA and βB, while it suppressed basal and hCG-induced follistatin expression in cultured follicle cells. These results, together with the evidence that EGF was predominantly expressed in the oocytes whereas EGFR was expressed in the follicle cells, strongly suggest that EGF is likely a potential paracrine/juxtacrine factor from the oocytes to regulate the function of the follicle cells.