Topical Losartan and Corticosteroid Additively Inhibit Corneal Stromal Myofibroblast Generation and Scarring Fibrosis After Alkali Burn Injury.
Topical Losartan and Corticosteroid Additively Inhibit Corneal Stromal Myofibroblast Generation and Scarring Fibrosis After Alkali Burn Injury.
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DOI：
10.1167/tvst.11.7.9
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发表时间：
2022-07-08
影响因子：
3
通讯作者：
Wilson, Steven E.
中科院分区：
文献类型：
--
作者：
Sampaio, Lycia Pedral;Hilgert, Guilherme S. L.;Shiju, Thomas Michael;Santhiago, Marcony R.;Wilson, Steven E.
关键词：
To evaluate the efficacy of losartan and prednisolone acetate in inhibiting corneal scarring fibrosis after alkali burn injury in rabbits. Sixteen New Zealand White rabbits were included. Alkali injuries were produced using 1N sodium hydroxide on a 5-mm diameter Whatman #1 filter paper for 1 minute. Four corneas in each group were treated six times per day for 1 month with 50 µL of (1) 0.2 mg/mL losartan in balanced salt solution (BSS), (2) 1% prednisolone acetate, (3) combined 0.2 mg/mL losartan and 1% prednisolone acetate, or (4) BSS. Area of opacity and total opacity were analyzed in standardized slit-lamp photos with ImageJ. Corneas in both groups were cryofixed in Optimal cutting temperature (OCT) compound at 1 month after surgery, and immunohistochemistry was performed for alpha-smooth muscle actin (α-SMA) and keratocan or transforming growth factor β1 and collagen type IV with ImageJ quantitation. Combined topical losartan and prednisolone acetate significantly decreased slit-lamp opacity area and intensity, as well as decreased stromal myofibroblast α-SMA area and intensity of staining per section and confined myofibroblasts to only the posterior stroma with repopulation of the anterior and mid-stroma with keratocan-positive keratocytes after 1 month of treatment. Corneal fibroblasts produced collagen type IV not associated with basement membranes, and this production was decreased by topical losartan. Combined topical losartan and prednisolone acetate decreased myofibroblast-associated fibrosis after corneal alkali burns that produced full-thickness injury, including corneal endothelial damage. Increased dosages and duration of treatment may further decrease scarring fibrosis. Topical losartan and prednisolone acetate decrease myofibroblast-mediated scarring fibrosis after corneal injury.
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影响因子：
17.8
作者：
Ljubimov AV;Saghizadeh M
通讯作者：
Saghizadeh M
角膜中的TGFβ1和TGFβ2蛋白，具有和不基质纤维化：顶部上皮生长因子屏障的延迟再生以及具有基质纤维化的角膜上的上皮地下膜。
影响因子：
3.4
作者：
de Oliveira RC;Tye G;Sampaio LP;Shiju TM;DeDreu J;Menko AS;Santhiago MR;Wilson SE
通讯作者：
Wilson SE
影响因子：
37.8
作者：
Geirsson, Arnar;Singh, Mansher;Tellides, George
通讯作者：
Tellides, George
影响因子：
82.9
作者：
Cohn, Ronald D.;van Erp, Christel;Dietz, Harry C.
通讯作者：
Dietz, Harry C.
影响因子：
3.4
作者：
Sampaio, Lycia Pedral;Shiju, Thomas Michael;Wilson, Steven E.
通讯作者：
Wilson, Steven E.