Lithium exposure enhances survival of NT2N cells (hNT neurons) in the hemiparkinsonian rat.
Lithium exposure enhances survival of NT2N cells (hNT neurons) in the hemiparkinsonian rat.
复制标题
锂暴露可增强偏侧帕金森病大鼠 NT2N 细胞（hNT 神经元）的存活率。
DOI：
10.1046/j.1460-9568.2002.02300.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Sanberg,PaulR
中科院分区：
文献类型：
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作者：
Willing,AlisonE;Zigova,Tanja;Milliken,Melissa;Poulos,Steve;Saporta,Samuel;McGrogan,Michael;Snable,Gary;Sanberg,PaulR
培养物中NTera 2/D1（或hNT神经元）的锂（Li+）处理增加了该细胞系中酪氨酸羟化酶（TH）的表达[Zigovaet al.，（1999）Exp.神经元，157，251-258]。目前尚不清楚这些Li+处理的细胞一旦移植到偏侧帕金森病大鼠的纹状体中是否保持TH表达。 hNT神经元用1 mmLiCl处理或不处理，然后移植到Sprague-道利大鼠的纹状体中。 一些细胞在培养物中暴露于锂24小时，而其他细胞仅在移植前短暂暴露（2-3小时）。 我们还检查了移植后动物的Li +治疗（食物中的0.24%w/w碳酸锂）是否有效增加神经元存活。 移植后一周，用4%多聚甲醛灌注动物，并在整个移植物的30 µm切片上进行免疫细胞化学。 人核基质抗原免疫染色表明，与所有其他组相比，用锂短暂处理的组中的细胞存活率显著更好。短暂暴露于锂也导致了THin原位的更大表达。神经元特异性烯醇化酶免疫组化显示，有广泛的纤维生长在所有组。这些结果表明，短暂的Li+暴露可将移植到偏侧帕金森病大鼠中的hNT神经元的存活率提高至60%以上，并将TH表达增加近三倍。
Lithium (Li +) treatment of NTera2/D1 (or hNT Neurons) in culture increases tyrosine hydroxylase (TH) expression in this cell‐line [Zigovaet al., (1999)Exp. Neurol.,157, 251–258]. It is not known if these Li + treated cells maintain TH expression once transplanted into the striatum of the hemiparkinsonian rats. hNT neurons were either treated with 1 mmLiCl or left untreated and then transplanted into the striatum of Sprague‐Dawley rats. Some cells were exposed to the lithium for 24 h in culture while others were exposed only briefly (2–3 h) just prior to transplantation. We also examined whether Li + treatment of the animal after transplantation (0.24% w/w lithium carbonate in chow) was effective in increasing neuronal survival. One week after transplantation, the animals were perfused with 4% paraformaldehyde and immunocytochemistry was performed on 30 µm sections through the transplant. Human nuclear matrix antigen immunostaining demonstrated that there was significantly better survival of cells in the group treated briefly with lithium compared to all other groups. Brief exposure to lithium resulted in a greater expression of THin situas well. Neuron specific enolase immunohistochemistry showed that there was extensive fibre outgrowth in all groups. These results suggest that brief Li + exposure may enhance survival to over 60% and increase TH expression of hNT Neurons transplanted in the hemiparkinsonian rat nearly three‐fold.